期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi在植物寄生线虫抗性研究中靶基因的选择策略 被引量：1

Target Genes Selection of RNAi in the Resistance to Plant-parasitic Nematodes
下载PDF
导出
摘要 RNA干扰(RNA interference,RNAi)是一种由dsRNA起始的转录后基因沉默机制。近年来,RNAi技术在植物抗线虫研究方面得到广泛的应用。随着线虫基因组测序工作的不断推进,越来越多的线虫基因被开发继而当作RNA干扰的靶基因,其中以寄生线虫的生长发育或寄生过程建立等所必需的基因作为RNAi的目的基因是防治线虫的新方法,也是培育抗性品种的新选择。文章介绍了在RNAi研究中,所选择的植物寄生线虫基因的种类及其研究进展。 RNAi（RNA interference） is a post-transcriptional gene silencing mechanism which is initiated by dsRNA.Recently,RNAi is widely applied in plant-parasitic nematode resistance.With the proceeding of nematode genome sequencing,more and more genes are explored as target genes for RNAi to improve the resistance to plant-parasitic nematodes.Among those genes,parasitism,development and mRNA metabolism related genes are suitable choices for RNAi and it is an alternative approach to engineer resistance to nematode.In this review,the selected genes and research progress are introduced.
作者 仲晓芳 刘晓冬 杨向东 邢少辰 袁翠平 董英山
机构地区 吉林省农业科学院农业生物技术研究所
出处 《大豆科学》 CAS CSCD 北大核心 2014年第4期599-602,共4页 Soybean Science
基金 国家自然科学基金(31000141) 中国博士后基金(2013M531004) 吉林省博士后基金 吉林省农业学院博士后基金
关键词 RNAI 植物寄生线虫 寄生 mRNA代谢 RNAi Plant-parasitic nematode Parastism mRNA metabolism
分类号 S432.45 [农业科学—植物病理学]
  • 相关文献

参考文献42

  • 1Bernstein E, Caudy A A, Hammond S M, et al. Role for a bidentate ribonuclease in the initiation step of RNA interference[ J]. Nature 2001,409:363-366.
  • 2Guo S, Kemphues K. Par-I, a gene required for establishing polari- ty in C. elegans embroys, encodes a putative Ser/Thr kinse that is asymmetrically distributed [ J ]. Cell, 1995,81:611 20.
  • 3Fire A, Xu S, Montgomery M K, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J]. Nature, 1998,391:806-811.
  • 4Kawasaki H, Taira K. Short hairpin type of dsRNA that are con- trolled by tRNA(Val) promoter significantly induce RNAi mediated gene silencing in the cytoplasm of human cells[J]. Nucleic Acids Research,2003,31 : 7002 -7007.
  • 5石智,符立梧.RNAi及其在肿瘤研究中的应用[J].生物化学与生物物理进展,2004,31(6):492-499. 被引量：11
  • 6Caplen N J, Fleenor J, Fire A, et aL dsRNA-mediated gene silen- cing in cultured Drosophila cells JJ. Gene,2000,252:95-105.
  • 7Palauqui J C,Elmayan T, Pollien J M, et aL Systemic acquired si- lencing: transgene-specific post-transcriptional silencing is trans- mitted by grafting from silenced stocks to non-silenced scions[ J ]. EMBO Journal, 1997,16:4738-4745.
  • 8Zamore P D,Tuschi T,Sharp P A,et al. RNAi:dsRNA directs the ATP dependent cleavage of mR.NA at 21 to 23 nuceotide intervals [J]. Ce11,1999,101:25-33.
  • 9Jones L, Ratcliff F, Baulcombe D C. RNA-directed transcriptional gene silencing in plants can be inherited independently of the RNA trigger and requires Metl for maintenance[ J]. Current Biology, 2001,11:747-757.
  • 10Winston W M, Molodowtich C,Hunter C P. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1 [ J ] Science, 2002,295 : 2459 -2459.

二级参考文献42

  • 1[1]Fire A,Xu S,Montgomery M K,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.Nature,1998,391(6669):806～811
  • 2[2]Hutvagner G,Zamore P D.RNAi:nature abhors a double-strand.Curr Opin Gene Devel,2002,12(2):225～232
  • 3[3]Ramaswamy G,Slack F J.SiRNA:A guide for RNA silencing.Chem Biol,2002,9(10):1053～1055
  • 4[4]Hannon G J.RNA interference.Nature,2002,418(6894):244～251
  • 5[5]Elbashir S M,Harborth J,Lendeckel W,et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.Nature,2001,411(6836):494～498
  • 6[6]MucManus M T,Sharp P A.Gene silencing in mammals by small interfering RNAs.Nature Reviews Genetics,2002,3(10):737～746
  • 7[7]Thompson J D.Applications of antisense and siRNAs during preclinical drug development.Drugdiscovery Today,2002,7(17):912～917
  • 8[8]Vickers T A,Koo S,Frank Bennett C,et al.Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents.J Biol Chem,2003,278(9):7108～7118
  • 9[9]Shuey D J,McCallus D E,Giordnno T.RNAi:gene-silencng in therapeutic intervention.Drugdiscovery Today,2002,7( 20 ):1040～1046
  • 10[10]Bridge A J,PebernaRd S,Ducraux A,et al.Induction of an interferon response by RNAi vectors in mammalian cells.Nature Genetics,2003,34(7):263～264

共引文献10

同被引文献7

引证文献1

二级引证文献1

大豆科学
2014年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部