牛蒡子苷元对人肝癌细胞HepG_2缝隙连接蛋白表达的影响

Intervention of Niubangzi aglycone on expression of HepG_2Cx

目的:观察牛蒡子苷元在体外对人肝癌细胞HepG2缝隙连接蛋白(Cx)表达的影响。方法:采用Western blot技术检测牛蒡子苷元对人肝癌细胞HepG2缝隙连接蛋白(Cx26/32/43)的表达。结果:Western blot检测,用国产多克隆Cx一抗和进口单克隆Cx一抗的实验均显示:牛蒡子苷元等能明显提高人肝癌细胞HepG2Cx32的表达,且有时间、浓度依赖性,氧化剂H2O2能抑制牛蒡子苷元此作用;但牛蒡子苷元对HepG2的Cx43表达影响24h不明显,48h能明显提高Cx43表达。结论:牛蒡子苷元能提高人肝癌细胞Cx表达,增强细胞间缝隙连接功能。

Objective： To observe intervention of Niubangzi aglycone on expression of HepG2Cx. Methods： Intervention of Niubangzi aglycone on expression of HepG2Cx26/32/43 was tested by Western blot. Results：Experiment of domestic polyclonal anti-Cx antibody and imported monoclonal anti-Cx antibody appeared that Niubangzi aglycone could obviously increase expression of HepG2Cx32 with time and concentration-dependent, oxidant H2O2 could inhibit the effect of Niubangzi aglycone; Niubangzi aglycone could not obviously affect expression of HepG2Cx43 within 24h, Niubangzi aglycone markedly improved expression of HepG2Cx43 within 48h with concentration-dependent. Conclusion：Niubangzi aglycone improved HepG2Cx expression and GJIC function.