葛根素聚氰基丙烯酸丁基酯纳米粒的制备及质量评价

Preparation and Quality Evaluation of Puerarin Polybutylcyanacrylate Nanoparticles

目的:制备葛根素聚氰基丙烯酸正丁酯纳米粒(P-PBCA),并对其进行质量评价。方法:以包封率和载药量为指标,采用正交设计综合评分法优化P-PBCA处方。结果:最佳工艺为葛根素质量20 mg、氰基丙烯酸正丁酯体积分数0.6%,pH为2.0,优化所得P-PBCA为球状,包封率为(78.13±7.42)%,载药量为(15.05±2.38)%;粒径(145.2±22.4)nm,Zeta电位(-28.7±1.1)mV。结论:正交设计综合评分法可用于P-PBCA的制备。

Objective： To prepare puerarin polybutylcyanacrylate nanoparticles （P-PBCA） and evaluate the quality. Methods： Encapsulation efficiency and drug loading as the indices, a comprehensive scoring method of orthogonal design was used to optimize the formula of P-PBCA. Results： The optimum preparation technology was as follows ： the amount of puerarin was 20mg, the volume frac- tion of BCA was 0.6% , and the pH value was 2.0. P-PBCA showed spherical shape with the encapsulation efficiency of （78.13 ± 7.42 ） %, drug loading of （ 15.05 ± 2.38 ） % , particle size of （ 145.2 ± 22.4） nm and Zeta potential of （ - 28.7 ± 1.1 ） inV. Conclu- sion： Comprehensive scoring method of orthogonal design is suitable for the optimization of P-PBCA formula.