HPLC法测定四维钙片中维生素C、B_1、B_2的含量

Determination of Vitamin C,Vitamin B_1 and Vitamin B_2 in Siweigai Tablets by HPLC

目的：建立高效液相色谱法测定四维钙片中维生素C、维生素B1和维生素B2含量的方法.方法：采用SHISEIDOC18（250 mm×4.6 mm,5μm）色谱柱,以0.05％磷酸溶液（含0.005 mol·L-1己烷磺酸钠）-乙腈（88：12）为流动相,流速：1.0ml· min-1,检测波长：244 nm,柱温：30℃,进样量：20μm.结果：维生素C、维生素B1和维生素B2的线性范围分别为7.52～90.24 μg· ml-1（r=1.000 0）,1.32 ～15.82 μg·ml-1（r=1.000 0）和1.27～15.24μg·ml-1 （r=1.000 0）;平均回收率分别为101.63％、99.77％和100.25％,RSD分别为1.51％、1.48％和1.84％（n=9）.结论：本法操作简便,结果准确、可靠,可用于四维钙片中维生素C、B1、B2的含量测定.

Objective： To establish an HPLC method for the determination of vitamin C, vitamin BI and vitamin B2 in Siweigai tab- lets. Methods： A SHISEIDO Cls column （ 250 mm x 4.6 mm,5 txm） was used for the separation with 0.05 % phosphoric acid （ contai- ning 0.005 mol · L-1 sodium hexanesulfonate）-aeetonitrile （88： 12）as the mobile phase, and the flow rate was 1.0 ml · min-1. The detection wavelength was 244nm, the column temperature was 30℃ and the injection volume was 20 μl. Results： The linear ranges of vitamin C, vitamin B1 and vitamin B2 was 7.52-90.24 μg· ml-1（ r = 1. 000 0）, 1.32-15.82 μg. ml-1（ r = 1. 000 0） and 1.27- 15.24 μg- ml-1 （r = 1.000 0）, respectively. The average recovery was 101.63% , 99.77% and 100.25% with RSD of 1.51%, 1.48% and 1.84% （ n = 9 ） , respectively. Conclusion： The method is simple and accurate in the analysis of Siweigai tablets.