RP-HPLC法测定前列癃闭通片中柚皮苷、新橙皮苷的含量

Determination of Naringin and Neohesperidin in Qianlielongbitong Tablets by RP-HPLC

目的：建立反相高效液相色谱测定前列癃闭通片中柚皮苷、新橙皮苷的含量测定方法.方法：色谱柱为WEL TECHHisep C18-T（250 mm×4.6 mm,5μm）;流动相：乙腈-水（20：80）（用磷酸调节pH值至3）;流速：1.0 ml·min-1;检测波长：283nm;柱温：25℃,进样量：10 μl.结果：柚皮苷进样量在0.143～3.570 μg内呈良好的线性关系;新橙皮苷进样量在0.164 ～4.110 μg内呈良好的线性关系.两者的平均回收率分别为99.06％、98.40％,RSD分别为1.64％、1.29％（n=6）.结论：该检测方法准确、可靠、重现性好,可用于前列癃闭通片中柚皮苷、新橙皮苷的含量测定.

Objective： To establish an RP-HPLC method for determining naringin and neohesperidin in Qianlielongbitong tablets. Methods： The column was a WEL TECH Hisep CIs-T （250 mm x4.6 mm, 5 μm） column with the temperature of 25℃, the mobile phase was acetonitrile-water （20： 80） （adjusting pH to 3 with phosphoric acid） with the flow rate of 1.0 ml · min-1. The detection wavelength was 283nm and the injection volume was 10μl. Results： The linear range of naringin was 0. 143-3. 570 μg. The linear range of neohesperidin was 0. 164-4.110 μg. The average recovery was 99.06% （RSD = 1.64% ） and 98.40% （ RSD = 1.29% ） （ n = 6）, respectively. Conclusion： The detection method is accurate, reliable and reproducible, and can be used in the determination of naring/n and neohesperidin in Qianlielongbitong tablets.