HPLC法测定藿香中胡薄荷酮的含量

Content Determination of Pulegone in Agastache Rugosa by RP-HPLC

目的：建立用高效液相色谱法测定藿香中胡薄荷酮含量的方法.方法：以甲醇-0.2％乙酸铵溶液（70：30）为流动相,Thermo BDS HYPERSIL C18（250 mm×4.6mm,5μm）为色谱柱,检测波长为252 nm,流速1.0ml·min-1.结果：胡薄荷酮进样量在0.211～2.111 μg的浓度范围内线性关系良好,r=0.999 9（n =5）,平均回收率为99.8％,RSD为1.2％.结论：该法结果准确、方法简便、分离度高,可用于藿香中胡薄荷酮的含量测定.

Objective ： To establish the method for the determination of pulegone in Agastache rugosus by RP-HPLC. Methods ： A Thermo BDS HYPERSIL Cls column （250 mm ×4.6 mm, 5 μm） was used. The mobile phase was composed of methanol-0.2% am- monium acetate（70： 30）. The flow rate was 1.0 ml · min- 1 and the detection wavelength was 252nm. Results： The linear relationship was good when pulegone was within the range of 0. 211-2.111 μg（ r = 0. 999 9, n = 5 ） and the average recovery was 99.8% （ RSD = 1.2% ）. Conclusion： The method is simple, rapid and accurate, which can be used as the content determination of pulegone in Agastache rugosa.