子痫前期患者胎盘HIF-1α和IL-6表达差异对胎儿发育的影响分析

Preeclampsia Patients with Placenta HIF-1α and IL-6 Expression Differences for Fetal Development Impact Analysis

目的:探讨缺氧抑制因子-1α(HIF-1α)和白细胞介素-6(IL-6)对子痫前期的发病机制及影响胎儿发育的作用。方法:其中足月择期行剖宫产的健康孕妇40例为正常对照组、子痫前期且胎儿发育正常的孕妇40例作为PE组、子痫前期且胎儿生长受限(FGR)的孕妇40例作为PE+FGR组。运用实时定量PCR(realtime-PCR)技术及蛋白印迹法(WB)检测各组孕妇胎盘组织中HIF-1α和IL-6 mRNA和蛋白的表达。结果:HIF-1α和IL-6 mRNA和蛋白的表达量:PE组和PE+FGR组与正常对照组比较,差异有统计学意义(P<0.01)。但PE组和PE+FGR组中HIF-1α和IL-6 mRNA及蛋白的表达比较,差异有统计学意义(P<0.05)。结论:胎盘性HIF-1α和IL-6对于导致子痫前期的发病及胎儿的正常发育具有重要的作用,为子痫前期的诊断及治疗提供了相关的应对策略。

Objective： To study the oxygen inhibition factor HIF-1α and IL-6 in the pathogenesis of preeclampsia and affect fetal development. Method： Including full line elective cesarean delivery of healthy pregnant women 40 cases as normal control group, preeclampsia, 40 cases of normal pregnant women and fetal development as PE group, pre-eclampsia and fetal growth restriction（FGR） 40 ~：ases of pregnant women as PE＋FGR group.Using real-time quantitative PCR（realtime-PCR） technology and protein imprinting method（WB） groups of pregnant women in the placenta tissue HIF-1α and IL-6 mRNA and protein expression.Result： HIF-1α and IL-6 mRNA and protein expression： PE and PE＋FGR group compared with normal control group, and extremely significant difference was statistically significant（P〈0.01）.But PE and PE＋FGR group of HIF-1α and IL-6 mRNA and protein expression, in contrast, a significant difference, with statistical significance（P〈0.05）.Conclusion： The placental HIF-1α and IL-6 for leading to the onset of preeclampsia and normal development of the fetus plays an important role, for the diagnosis and treatment of preeclampsia provides relevant countermeasure.