人不同转移潜能前列腺癌细胞系的放射敏感性异质性及其机理观察

Study on the Radiosensitivity Heterogeneity of Different Metastatic Potential Cell Lines of Human Prostate Cancer and Its Mechanism

目的:研究人不同转移潜能前列腺癌细胞系PC-3M-2B4(低转移潜能)和PC-3M-IE8(高转移潜能)放射敏感性异质性及其机理。方法:通过流式细胞仪、荧光显微镜、电镜和Western-blot检测PC-3M-2B4和PC-3M-IE8凋亡能力的差别及凋亡相关蛋白Bcl-2、Bax、Caspase-3表达的差异,来证实人不同转移潜能前列腺癌细胞与放射敏感性的关系。结果:PC-3M-2B4和PC-3M-IE8两种细胞的凋亡率都随着照射后时间的延长而增加,但PC-3M-2B4比PC-3M-IE8凋亡率高,且差异有统计学意义(P<0.05);抗凋亡蛋白Bcl-2表达时间依赖性递减,而促凋亡蛋白Bax和Caspase-3表达明显升高。结论:人不同转移潜能前列腺癌细胞系,其转移能力与放射敏感性有关,转移能力低的对放射敏感性高。

Objective： To study the radiosensitivity heterogeneity of different metastatic potenhal cell hnes ot human prostate cancer PC-3M-2B4（low metastatic potential） and PC-3M-lE8（high metastatic potential） and Its mechanism.Method： To detect differences in PC-3M-2B4 and PC-3M-IE8 apoptotic ability, to detect the differences of apoptotie ability in PC-3M-2B4 and PC-3M-IE8 and the expression of apoptosis-related proteins Bcl-2, Bax and Caspase-3 by flow cytometry, fluorescence microscopy, electron microscopy and Western-blot.Result： The apoptosis rate of two cell PC-3M-2B4 and PC-3M-IE81 were irradiated with the extension of time increases.Compared to PC-3M-IE8, the apoptosis rate of PC-3M-2B4 was statistically significant（P〈0.05）.The expression of auti-apoptotie protein Bcl-2 decline with time-dependent, while the expression of pro-apoptotic proteins Bax and Caspase-3 was significantly increased. Conclusion： Human prostate cancer cell lines with different metastatic potential, its ability of transfer has related to the and radiosensitivity, those who have low transfer ability with high radiosensitivity.