pH值对剩余污泥中氮、磷溶出及菌群组成的影响

Impact of p H on Dissolution of Nitrogen and Phosphorus and Community Composition of Excess Sludge

为了研究剩余污泥水解酸化过程中氮、磷的溶出规律及菌群组成，分别控制pH值为5-12及不调节，测定各条件下溶出的NH4-N和PO3- 4-P浓度，并采用PCR—DGGE技术分析剩余污泥中的菌群组成。结果表明：对剩余污泥的pH值进行调节，能够提高氮、磷的溶出量，且pH值为9—11条件下的NH4＋-N溶出量远高于其他条件下的。与NH4＋-N的溶出不同，酸性条件更有利于PO3-4-P的溶出。不同污泥样品具有不同的DGGE指纹图谱特征，即菌群组成不同，这在一定程度上影响着剩余污泥水解酸化过程中氮、磷的溶出。

To study the dissolution of nitrogen and phosphorus and bacterial community composi-tion during the hydrolysis and acidification of excess sludge, the concentrations of NH4＋ - N and PO3- 4-P dissolved from excess sludge were measured when pH was 5 to 12 and without adjusting. The bacterial community composition of excess sludge was analyzed by PCR-DGGE. The results showed that adjusting of pH could realize a significant increase in dissolution of nitrogen and phosphorus. For the dis-solution of NH4 - N, pH 9 to 11 was better than other environments. Unlike the dissolution of NH4 - N, acid environment was more beneficial to dissolution of PO3-4 - P. There were differences in DGGE profilesof different sludge samples, namely there were differences in the bacterial community compositions, which affected the dissolution of nitrogen and phosphorus during the hydrolysis and acidification of excesssludge to some extent.