玉米芯水解制木糖过程中氛态氬类成分研究

Analysis of the ammonia nitrogen in the hydrolysis processing of corncob for xylose

分别利用微量凯氏定氮法检测了玉米芯及木糖生产过程中物料、副产物的总氨态氮含量;利用考马斯亮蓝染色法检测了木糖生产过程中物料的蛋白质含量;利用SDS-PAGE检测了木糖生产过程中蛋白质的相对分子质量分布变化情况。结果显示,玉米芯中总氨态氮含量约为2.44%(干基);活性炭对物料中的总氨态氮和蛋白质有一定的脱除作用;离子交换树脂相对脱除作用不明显;玉米芯水解液中蛋白质相对分子质量主要分为66.4 KDa附近和小于14.3 KDa的两个区域,通过活性炭有效脱除的是低分子量的蛋白质,相对分子质量为66.4 KDa的蛋白质无法在活性炭脱色和离子交换工序有效脱除。

In this paper, the con tents of total ammonia nitrogen （TAN） in the hydrolysis processing of corncob for xylose were tested by Kjeldahl method; contrast test of the protein contents was done by Coomassie brilliant blue R 250 ; the molecular weight of protein were tested by SDS - PAGE. The TAN content of corncob is 2.44 % （ dry basis）. Activated carbon plays a very significant role in removing protein and TAN of corncob hydrolysate. Compare with activa- ted carbon, ion - exchange resin cannot adsorb protein and TAN effectively. The molecular weight of protein in corncob hydrolysate is focus at 66. 4KDa and below 14. 3 KI）a. The small molecule protein （below 14. 3 KDa） can remove by activated carbon effectively. The bigger protein （66.4 KDa） cannot remove by activated carbon or Ion - exchange res- in