摘要
目的:国标方法采用纸色谱法进行诱惑红的分离、定性,分光光度法定量,此方法比较繁锁,不易操作,本实验通过对国标检测方法的改进,建立了饮料中诱惑红的高效液相色谱分析方法,采用液相色谱分离、定性、定量,简化了实验步骤,缩短了检测时间。方法:样品经聚酰胺粉吸附,甲醇一甲酸溶液洗去天然色素,用乙醇一氨水一水混合溶液(7:2:1)解析,100℃水浴蒸至1~2mL,定容至lOmL后经C18液相色谱柱梯度洗脱分离,采用二极管阵列检测器检测,外标法定量。结果:诱惑红的检出限为2.5mg/kg,标准曲线相关系数0.9999,回收率均大于93%,变异系数小于2.8%。结论:该方法回收率高、稳定性好,适用于饮料中诱惑红检测。
Objective : separating out allura red using paper chromatography of the national standard, spectrophotometer quantification. This method is cumbersome and difficuh to operate, through the improvement of national standards es- tablished high performance liquid chromatography analytical method. In wine, using liquid chromatography separation simplified the experimental steps and shortened detection time. Methods: Samples of polyamide adsorption, methanol and formic acid solution were used to wash away the natural pigment, eluted with an alcohol - ammonia - water solution (7 : 2 : 1) in a 100℃ water bath and steam to 1 ~2mL, constant volume to 10mL and gradient elution separation by C18 liquid chromatography column, using a diode array detector and external standard method quantitative. Results: The detection limit is 2. 5mg/kg, the correlation coefficient of the standard curve is 0. 9999, the recovery rate was more than 93%, the RSD less than 2. 8%. Conclusion: the method has a high recovery rate and good stability, which makes it suitable for allura red detection in drinks.
出处
《中国食品添加剂》
CAS
北大核心
2014年第5期195-198,共4页
China Food Additives