饮料中诱惑红检测方法的改进

Improvement on the method of allura red determination in drink

目的：国标方法采用纸色谱法进行诱惑红的分离、定性，分光光度法定量，此方法比较繁锁，不易操作，本实验通过对国标检测方法的改进，建立了饮料中诱惑红的高效液相色谱分析方法，采用液相色谱分离、定性、定量，简化了实验步骤，缩短了检测时间。方法：样品经聚酰胺粉吸附，甲醇一甲酸溶液洗去天然色素，用乙醇一氨水一水混合溶液（7：2：1）解析，100℃水浴蒸至1～2mL，定容至lOmL后经C18液相色谱柱梯度洗脱分离，采用二极管阵列检测器检测，外标法定量。结果：诱惑红的检出限为2．5mg／kg，标准曲线相关系数0．9999，回收率均大于93％，变异系数小于2．8％。结论：该方法回收率高、稳定性好，适用于饮料中诱惑红检测。

Objective ： separating out allura red using paper chromatography of the national standard, spectrophotometer quantification. This method is cumbersome and difficuh to operate, through the improvement of national standards es- tablished high performance liquid chromatography analytical method. In wine, using liquid chromatography separation simplified the experimental steps and shortened detection time. Methods： Samples of polyamide adsorption, methanol and formic acid solution were used to wash away the natural pigment, eluted with an alcohol - ammonia - water solution （7 ： 2 ： 1） in a 100℃ water bath and steam to 1 ～2mL, constant volume to 10mL and gradient elution separation by C18 liquid chromatography column, using a diode array detector and external standard method quantitative. Results： The detection limit is 2. 5mg/kg, the correlation coefficient of the standard curve is 0. 9999, the recovery rate was more than 93%, the RSD less than 2. 8%. Conclusion： the method has a high recovery rate and good stability, which makes it suitable for allura red detection in drinks.