摘要
目的从胁迫的角度为微重力影响质膜的结构和功能提供直接的证据。方法以回转器模拟微重力 ,根据膜脂过氧化产物丙二醛 (MDA)与硫代巴比妥酸 (TBA)的定量反应测定MDA含量 ,用电导仪检测细胞电解质的外渗率。结果在模拟微重力胁迫下 ,两种蓝藻———鱼腥藻和集球藻的膜脂过氧化产物———丙二醛 (MDA)不断积累 ,同时细胞膜的透性相应增大。结论模拟微重力与其它胁迫因子有着相同的共性 ;对没有特殊重力感受结构的单细胞而言 。
Objective To provide direct evidences for effects of microgravity on structure and function of plasma membrane. Method Malondialdehyde (MDA) content was examined on the basis of quantitative reaction of both MDA and thiobarbituric acid (TBA), and electrolyte leaking was determined with conductometer model DDS 11A. Result Experiments showed that under simulated microgravity, lipid peroxidation became stronger and the content of MDA increased. Meanwhile, the membrane permeability increased in cells of two microalgae: Anabaena sp PCC7120 and Synechococcus 7942. Conclusion Our results suggest that there is some commonness between microgravity stress and certain other environmental stresses. And cellular membrane might be the site of perception of gravity in unicells without special gravity sensitive structure, such as alga cells.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2002年第4期270-272,共3页
Space Medicine & Medical Engineering
基金
国家载人航天工程项目
中国科学院水生生物研究所创新领域前沿项目资助 (2 2 0 3 16)
