树突细胞激活的肿瘤浸润性淋巴细胞抗肝癌免疫活性的研究

Dendritic Cells Affect Anti-hepatocarcinoma Activity of Tumor Infiltrating Lymphocytes

目的 探讨树突细胞 (DC)激活的肿瘤浸润性淋巴细胞 (TIL)体外对肝癌细胞的杀伤活性。方法 从肝癌患者外周血获取DC ,应用粒 /巨噬细胞集落刺激因子 (GM -CSF)、白细胞介素 -4 (IL -4 )和肿瘤抗原激活DC ,然后用DC激活TIL ,观察TIL在体外对自体肝癌细胞和Hep3B细胞的杀伤活性。 结果 DC激活的TIL对自体肝癌细胞具有很强的杀伤活性 ,杀伤率为89 .39%± 3.0 5 %,明显高于未经DC激活的TIL、DC激活的T淋巴细胞和未经DC激活的T淋巴细胞对自体肝癌细胞的杀伤率(分别为 5 5 .2 3%± 1.5 3%、5 4.89%± 1.48%和 3.6 5 %± 0 .2 6 %)。而它们对Hep3B细胞的杀伤活性则相对较低。 结论 肝癌患者外周血DC能诱导TIL产生高效而特异的抗肝癌免疫活性。

Objective To study the killing activity of tumor infiltrating lymphocytes(TIL) stimulated by dendritic cells(DC) on hepatocarcinoma cells in vitro.Methods Isolated from the peripheral blood of patients′ with hepatocarcinoma,DC were stimulated by granulocyte/macrophage colony stimulating factor(GM-CSF),interleukin-4(IL-4) and tumor antigen.Then TIL were stimulated by DC and their killing activity on autogenous hepatocarcinoma cells and Hep3B cells in vitro were observed.Results TIL stimulated by DC had very high killing activity on autogenous hepatocarcinoma cells(89.39%±3.05%).It was obviously higher compared with TIL without DC stimulated or T lymphocytes stimulated by DC or not on autogenous hepatocarcinoma cells(killing rate:55.23%±1.53%,54.89%±1.48% and 3.65%±0.26%,respectively).Their killing activity on Hep3B cells was also lower.Conclusion The results indicate that DC from patients with hepatocarcinoma may induce efficient and specific anti-hepatocarcinoma immune response.