黑穗醋栗AsA含量及其代谢酶活性差异分析

Ascorbic acid content and difference of related enzyme activities in fruit of blackcurrant

[目的]分析黑穗醋栗不同品种抗坏血酸（AsA）含量及其合成代谢相关酶活性的差异,为研究黑穗醋栗果实AsA积累规律和选育优良黑穗醋栗品种提供参考依据.[方法]采用液相色谱测定30个黑穗醋栗品种的AsA含量,选择含量差异较大、生产中广泛应用的8个品种,测定其AsA合成代谢相关酶的活性,并比较分析不同品种间的差异.[结果]30个黑穗醋栗品种果实AsA含量为144.79～329.71 mg/100 gFW,呈现出明显的多样性.AsA含量较高的品种（路德克、寒丰、亚德）其抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHRA）、单脱氢抗坏血酸还原酶（MDHRA）活性较高,而AsA含量较低的品种（利桑佳、黑丰、奥依宾）还原酶活性相对较低;AsA含量与MDHAR、DHAR活性呈极显著正相关,相关系数分别为0.982和0.951,与（APX）活性呈显著正相关,相关系数为0.826.[结论]不同品种黑穗醋栗AsA含量及AsA合成相关酶活性差异较大,可作黑穗醋栗品种分类和品种选育的参考指标.

[Objective]The diversity of ascorbic acid content and the differences of related enzyme activity in synthetic metabolism of different blackcurrant varieties were studied in order to provide scientific references for breeding fine varieties and identifying the accumulation law of ascorbic acid （AsA） of blackcurrant.[Method]The fruit AsA content of 30 varieties was determined using HPLC,then 8 varieties which showed great differences in AsA content and were widely used in the production were selected to determine the enzyme activity in synthesis metabolism,and differences of enzyme activity were analyzed among different varieties.[Result]The fruit AsA content was 144.79-345.25 mg/100 g FW,and the diversity was obvious.The varieties （Ludeke,Hanfeng） with higher AsA content showed higher enzyme activity of ascorbic acid peroxidase （APX）,dehydrogenation ascorbic acid reductase （DHAR） and single dehydrogenation ascorbic acid reductase （MDHAR）.The AsA content,DHAR and MDHAR had extremely significant positive correlation,and the correlation coefficient was 0.952 and 0.983,respectively.The varieties （Lisangjia,Haifeng） with lower AsA content showed lower enzyme activity.The AsA content and APX had significantly positive correlation,and the correlation coefficient was 0.826.[Conclusion]Vast differences of AsA content and enzyme activity are observed in different blackcurrant varieties,which provides references for breeding and species classification of blackcurrant.