摘要
采用二次正交旋转组合设计对顶果木(Acrocarpus fraxinifolius)增殖培养基进行优化,建立增殖倍数(Y)对培养基中Ca2+浓度(X1)、6-BA浓度(X2)及IBA浓度(X3)的正交回归模型:Y=2.416-0.172X1-0.255X2+0.183X12-0.211X22+0.162X32+0.331X1X2。从模型可知,当Ca2+浓度为255 mg/L,6-BA浓度为0.76 mg/L,IBA浓度为0.16 mg/L时,增殖倍数达最大值4.45。模型预测值与实际验证结果较接近。
The multiplication medium of Acrocarpus fraxinifolius was optimized with quadratic orthogonal rotation combination design.The quadratic orthogonal regression model of multiplication rate （Y） to three factors including concentration of Ca2＋ （X1）,6-BA（X2） and IBA（X3） was established as Y =2.420-0.172X1-0.255X2 ＋ 0.184X12-0.211X22 ＋ 0.162X32 ＋ 0.331X1X2.It was concluded from the model that when the concentration of Ca2＋ （X1）,6-BA（X2） and IBA（X3） was 255 mg/L,0.76 mg/L and 0.16 mg/L respectively,the r axirum rultiplication rate （Y） was 4.45 which was close to the experiment result.
出处
《广东林业科技》
2014年第4期58-61,共4页
Forestry Science and Technology of Guangdong Province
基金
广东省林业科技创新专项"顶果木引种和高效栽培技术研究与示范"(2010KJCX005)
关键词
顶果木
组织培养
增殖
二次正交旋转组合设计
Acrocarpus fraxinifolius
tissue culture
proliferation
quadratic orthogonal rotation combination design
