蝙蝠葛根茎愈伤组织培养及试管苗分化的研究

Study on the Callus Culture and Differentiation of Tube Seeding of Menispermum dauricum DC

为保护蝙蝠葛的野生资源,该研究以嫩茎为材料,进行了愈伤组织生长、愈伤组织分化培养,生长芽生根及试管苗移栽和定植的研究。结果证明:MS+琼脂0.46%+蔗糖37 g/L+ZT 0.5 mg/L+La(NO3)3.6H2O 0.3 mg/L+2,4-D 2.4 mg/L是嫩根茎段愈伤组织生长培养的适宜培养基;1/2 MS+AgN030.6 40 g/L+琼脂0.47%+蔗糖3.3%+NAA 0.075 mg/L+BA 0.45 mg/L是愈伤组织分化培养的适宜培养基;White+琼脂0.48%+ABT2号0.6 mg/L+蔗糖1.4%+IAA 0.1 mg/L是生长芽生根培养的适宜培养基;试管苗移栽成活率为89.2%;定植成活率为98.9%。定植的试管苗次年正常开花结果,保持了野生蝙蝠葛的植物学性状。

In order to preserve the wild resource of Menispermum dauricum DC.,the younger stem was used as material to study the growth and differentiation of the callus,growth rooting of buds,transplanting and colonizing testtube seeding. The results demonstrated that MS ＋ agar 0. 46 % ＋ sucrose 37 g /L ＋ ZT 0. 5 mg /L ＋ La（ NO3）3.6H2 O 0. 3 mg /L ＋ 2,4-D 2. 4 mg /L was suitable medium for callus culture,1 /2MS ＋ AgN030. 6 40 g /L ＋ agar 0. 47% ＋ sucrose 3. 3 % ＋ NAA 0. 075 mg /L ＋ BA 0. 45 mg /L was suitable for differentiation of callus culture and White＋ agar 0. 48 % ＋ ABT2#0. 6 mg /L ＋ sucrose 1. 4 % ＋ IAA 0. 1 mg /L was appropriate for growth rooting of buds. The survival rate for transplanting test-tube seeding was 89. 2 % and the colonization survival rate was 98. 9 %. The testtube seeding had flower and fruit in the following year and maintained all biological traits of wild plant.