沸石培养基对大羽藓(Thuidium cymbifolium)配子体生长的影响

Effects of zeolite medium on growth of Thuidium cymbifolium gametophyte

以大羽藓(Thuidium cymbifolium)的配子体为试验材料进行组织培养,通过观察外观变化并定期记录其一级分枝长度、一级分枝个数等指标,研究了沸石培养基对大羽藓配子体生长的影响。结果表明:0.2%NaClO消毒60 s效果最好,以沸石为培养基7 d后污染率、死亡率均为0,存活率100%;沸石培养基促进大羽藓配子体的生长受沸石浓度、培养基pH值、琼脂浓度、蔗糖浓度的影响。沸石培养基促进大羽藓配子体生长的最适条件是:沸石浓度2.0%、培养基pH值7.5、琼脂浓度1.0%。在此条件下,大羽藓30 d后一级分枝长度为3.44 cm、一级分枝数量达2.70个。

Thuidium cymbifolium gametophyte was cultured as experimental material .The effects of zeolite medium on the growth index of Thuidium cymbifolium gametophyte , such as the primary branch length and primary branch number were investigated .The results showed that the best disinfection method was 0.2%NaClO for 60 s, and the contamination rate, mortality rate on zeolite medium was 0, while the survival rate was 100% after 7 d.It was affected by the zeolite concentration , pH value of medium , agar and sucrose concentration to promote the growth of Thuidium cymbifolium gametophyte on zeolite medium .The optimal condition for the growth of Thuidium cymbifolium gametophyte on zeolite medium was shown as follows：zeolite concentration 2.0%, pH value 7.5 , agar concentration 1.0%.The primary branch length was 3.44 cm, and the number of primary branches was up to 2.70 after 30 d under that condition .