响应面分析法优化超声提取连翘花黄色素工艺研究

Optimization on ultrasonic extraction of total yellow pigment from Forsythia suspensa Vahl. based on response surface methodology

以黄色素吸光度为衡量指标,在单因素试验基础上,运用Box-Behnken中心组合采用四因子三水平试验模型,建立回归方程,以响应面分析法(RSM)对连翘花黄色素的提取工艺进行优化。结果表明,以料液比1∶28(V/m),乙醇浓度96%,超声时间31 min,超声温度72℃为提取条件,实际平均色素吸光度为0.735 5,理论值为0.755 7,二者相差较小。

The absorbance of yellow pigment was used as the measurement indicator .The regression equation was established by using four factors-three levels experimental model of Box-Behnken central composite on the basis of single factor test.The extraction method of yellow pigment from Forsythia suspensa Vahl.was optimized by response surface methodology .The results showed that optimal extraction conditions were as follows： solid-liquid ratio of 1∶28, alcohol concentration of 96%, ultrasonic time of 31 min, ultrasonic temperature of 72℃.The practical value was 0.7355 , which was close to the model prediction extraction absorbance of 0.7557 .