联苯菊酯降解菌筛选及其反应条件优化

Screening of bifenthrin degrading bacteria and optimization of its reaction conditions

采用富集培养的方法,从农药厂废水处理池污泥中分离出一株对联苯菊酯有较强降解能力的菌株TS-1,并研究了初始pH、培养温度、联苯菊酯初始质量浓度、摇床转速、接种量、外加碳源质量分数对该菌株联苯菊酯降解能力的影响。结果表明:TS-1菌为革兰氏阴性杆菌,能以联苯菊酯为唯一碳源生长,其降解联苯菊酯的最佳反应条件为:pH7.0,培养温度30℃,联苯菊酯初浓度200 mg·L-1,摇床转速150 r·min-1,接种量10%,外加碳源葡萄糖为100 mg·L-1。

A bacterial strain capable of degrading bifenthrin was isolated from wastewater treatment sludges , and was designated as TS-1.This strain was gram negative bacilli , and it could take bifenthrin as its sole carbon source .In order to optimize the reaction condition for bifenthrin degradation , the effects of initial pH ,culture temperature ,initial bifenthrin concentration ,rotate speed of shaker ,carbon source concentration , inoculum size of bifenthrin-degradation strains were examined .It was shown that the optimal condition for bifenthrin degradation by TS-1 was as follows：pH 7.0, 30℃, 200 mg· L^-1 initial bifenthrin, 150 r· min^-1 shaking speed, 10%inoculum size, 100 mg· L^-1 glucose as exogenous carbon source .