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热休克蛋白10与锰致神经细胞凋亡关系的研究 被引量:1

Roles of heat shock protein 10 in apoptosis of SH-SY5Y cells caused by manganese
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摘要 目的探讨SH-SY5Y细胞经锰染毒后Hsp10表达与细胞活力、凋亡率之间的关系。方法将受试SH-SY5Y细胞分为对照组和实验组,A组(0.9%NaCl)作为对照组,B组(Mn2+0.5 mmol/L)、C组(Mn2+1.0 mmol/L)、D组(Mn2+2.0mmol/L)和E组(Mn2+4.0 mmol/L)为实验组。观察锰不同剂量染毒12 h后SH-SY5Y细胞活力、凋亡率的变化;实时荧光定量PCR(RT-PCR)检测Hsp10 mRNA的相对表达水平,Western blot检测Hsp10蛋白表达情况。结果细胞染毒以后,与对照组比较,不同浓度Mn2+组的细胞活力显著下降(P<0.05),分别为对照组的92.93%,87.28%,79.53%和73.67%,与对照组相比差异有统计学意义(P<0.05);各浓度Mn2+组凋亡率呈明显的剂量反应关系,随Mn2+染毒浓度增加而上升;Hsp10基因的表达水平(mRNA和蛋白)也呈显著的剂量反应关系,各浓度组Hsp10 mRNA的相对表达分别是对照组的0.95,0.84,0.63,0.46倍,各浓度组Hsp10蛋白含量与A组相比差异有统计学意义(P<0.05)。结论 Mn可引起SH-SY5Y细胞活力逐渐下降,凋亡率逐渐上升,Hsp10表达水平(mRNA和蛋白)下降,提示Hsp10可能在Mn诱导细胞凋亡过程中发挥重要的作用。 Objective To explore the relationship between cell viability,apoptosis rate and heat shock protein 10 (Hsp10) expression after SH-SY5Y cells exposure to manganese(Mn2+).Methods SH-SY5Y cells were divided into control group (group A,normal saline,0.9% NaCl) and experimental groups (group B:Mn2 + 0.5 mmol/L,group C:Mn2 + 1.0 mmol/L,group D:Mn2 + 2.0 mmol/L,group E:Mn2+ 4.0 mmol/L).After SH-SY5Y cells exposed to Mn2+ for 12 h,the cell viability and apoptosis rate in each group was measured by MTT method,and expression of Hsp10 mRNA and protein was detected by RT-PCR and Western blot.Results Compared to control group,the cell viability significantly decreased in each experimental group (P < 0.05),which was 92.93%,87.28%,79.53% and 73.67%,respectively.The apoptosis rate increased in a dose-dependent manner after exposure to Mn2+.Similarly,the mRNA and protein expression of Hsp10 presented a notably dose-dependent decrease.Hsp10 mRNA level was 0.95,0.84,0.63,0.46 folds of the control in groups B,C,D,E and the Hsp10 protein expression was significantly lower than in control group(P <0.01).Conclusion Manganese can attenuate the cell viability and Hsp10 expression (mRNA and protein) but elevate the rate of apoptosis,which suggest that Hsp10 might play an important role in Mn-induced apoptosis.
出处 《山西医科大学学报》 CAS 2014年第8期683-687,共5页 Journal of Shanxi Medical University
基金 国家自然科学基金项目(81172640) 北京市教育委员会科技发展计划面上基金资助项目(KM201110025012) 北京市自然科学基金资助项目(7132026)
关键词 热休克蛋白10 细胞凋亡 SH-SY5Y细胞 manganese heat shock protein 10 apoptosis SH-SY5Y cell
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