EIF5A2基因在不同发育时期人胎盘绒毛膜中的定位表达及意义

Expression and localization of EIF5A2 in human placental chorion at different developmental stages

目的探讨EIF5A2(eukaryotic transla-tion initiation factor 5A2)癌基因在人胎盘绒毛膜中表达及其定位。方法收集孕早期(8±周)绒毛膜、孕中期(20±周)及足月(38±周)胎盘组织,应用RT-PCR和Western blot及免疫组织化学等方法,从分子水平及蛋白水平检测EIF5A2在不同时段胎盘组织中的表达及定位。结果 RT-PCR及Western blot检测结果显示各期胎盘组织中均有EIF5A2 mRNA及蛋白表达,孕中期达峰值,足月胎盘EIF5A2表达显著下降(P<0.05);免疫组化结果显示孕早期绒毛膜中EIF5A2定位表达于合体滋养层及细胞滋养层细胞胞质,孕中期胎盘组织中EIF5A2主要表达于细胞滋养层,合体滋养层表达较弱,分娩期胎盘部分合体滋养层细胞内可见微弱的EIF5A2表达。结论 EIF5A2在不同时段人绒毛膜、胎盘组织中均有表达,定位于胎盘绒毛膜合体滋养层及细胞滋养层细胞胞质,其表达丰度及优势表达细胞类别在不同孕期存在差异。

Objective To explore the expression and localization of oncogene EIF5A2 in human placental chorion at different stages.Methods Immunohistochemistry（IHC） staining,reverse transcription-PCR（RT-PCR） and Western blot were used to investigate the expression and localization of EIF5A2 in human placental chorion.Results RT-PCR and Western blot showed that EIF5A2 mRNA and protein expression were found at different stages in human placenta,and they were the highest in the midtrimester and the lowest in the term placenta（P 〈 0.05）. Immunohistochemistry showed that EIF5A2 expressed in cytoplasm of both syncytiotrophoblast and cytotrophoblast in the first trimester.In the midtrimester,EIF5A2 mainly espressed in cytotrophoblast,and weakly in syncytiotrophoblast.In the term placenta,only in part of syncytiotrophoblast was there weak EIF5A2 expression.Conclusion EIF5A2 expresses at different stages in human placenta,which is localized in both syncytiotrophoblast and cytotrophoblast,and the expression level and the cells of predominant expression are different at developmental stages.