组织块法和酶消化法培养大鼠颌下腺细胞的比较研究

Comparative Study of Tissue-explant Technique and Enzyme Digestion Method of Culturing Rat Submandibular Gland Cells

应用组织块法和酶消化法进行原代大鼠颌下腺细胞(rat submandibular gland cells,RSGCs)分离培养,通过免疫细胞化学法检测细胞角蛋白-8的表达,经DAB染色均呈阳性,提示培养细胞为上皮细胞来源。在倒置显微镜下观察细胞数量、生长情况和生长周期,两种方法所培养的原代大鼠颌下腺细胞均生长良好,相互聚集形成腺泡、腺管样结构或呈铺路石状。其中酶消化法所培养细胞贴壁较好,生长周期短,细胞数量更多。提示酶消化法分离培养较组织块法更易获得大量RSGCs,为以颌下腺细胞作为实验载体的涎腺非肿瘤性疾病的研究奠定了实验基础。

Primary RSGCs were obtained via two methods： tissue-explant technique and enzyme digestion method. The cytokeratin-8 immunocytochemistry staining was positive, which indicated epithelial cell. Cell number, cell growth, and cell cycle were observed by inverted microscope. The paving stone shaped cells grew well in vitro, while enzyme digestion methods can get better growth, shorter cycle and larger production. Enzyme digestion method is easier to purify plentiful RSGCs with normal functions than the tissue-explant technique.