乌司他丁对脂多糖所致内毒素血症大鼠胃肠黏膜循环的影响

Effect of ulinastatin on the gastrointestnal circulation in rats with sepsis induced by LPS

目的探讨乌司他丁(ulinastatin,UTI)对脂多糖所致内毒素血症大鼠胃肠黏膜循环的影响。方法 30只雄性Wistar大鼠随机分成3组,正常对照组(NC组)、脂多糖(Lipopolysaccharide,LPS)处理组(LPS组)和乌司他丁处理组(UTI组),每组10只。正常对照组只麻醉不处理,脂多糖组和乌司他丁组经腹腔注射脂多糖5 mg/kg,乌司他丁组于脂多糖注射前经尾静脉静注乌司他丁50 000 U/kg。分别于处理前、脂多糖注射后3、6和12 h检测胃黏膜pH(pHi)值,同时经股静脉抽血检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)。采用SPSS 13.0软件进行统计学分析,结果以均数±标准差(x珋±s)表示。各时间点组间比较采用单因素方差分析,P<0.05为差异有统计学意义。结果经脂多糖处理后,大鼠3 h后血浆TNF-α和IL-6含量即开始逐渐升高,胃黏膜pH逐渐降低;与脂多糖组比,乌司他丁组在T3、T6和T12时间点TNF-α含量明显降低(T3:P=0.044;T6:P=0.035;T12:P=0.033),IL-6含量明显降低(T3:P=0.041;T6:P=0.022;T12:P=0.024),胃黏膜pH明显升高(T3:P=0.003;T6:P=0.028;T12:P=0.012)。结论乌司他丁可降低内毒素血症大鼠外周血TNF-α和IL-6浓度,增加胃黏膜pH值,改善胃肠黏膜灌注。

Objective To explore the effect of ulinastatin on the gastrointestnal circulation in rats with sepsis induced by LPS.Methods Thirty adult Wistar rats were randomly divided into three groups （n=10）：normal control group （group NC）,LPS group （group LPS） and ulinastatin group （group UTI）.Group NC received only anesthesia.The rats in group LPS and group UTI received 5 mg/kg of LPS.5 minutes before the injection of LPS, rats in the group UTI received 50 000U/kg of ulinastatin.The blood of the femoral vein was collected to determine the levels of TNF-αand IL-6, and the pH of gastric mucosa was measured at 0, 3, 6 and 12 hours after processing .The experimental results are expressed as mean ±SD.Statistical analyses were carried out by using SPSS 13.0.One-way ANOVA was used to compare the results of the groups .Differences were considered to be statistically significant when P〈0.05.Results After 3 hours of LPS treatment , plasma TNF-αand IL-6 levels of the rats began to rise gradually , the pH of gastric mucosa gradually reduced . Compared with group LPS, the levels of TNF-αin group UTI were significantly reduced （ T3：P=0.044;T6：P=0.035;T12：P=0.033）, the levels of IL-6 were significantly reduced（T3：P=0.041;T6：P=0.022;T12：P=0.024）, and the pH of gastric mucosa was significantly increased （T3：P=0.003;T6：P=0.028;T12：P=0.012）.Conclusion Ulinastatin could improve systemic inflammatory response syndrome and gastrointestinal blood perfusion in rats with sepsis , with reduced TNF-αand IL-6 and increased pH .