ICP-AES法测定肠内营养乳剂(TPF-D)中4种元素的含量

Content determination of four elements in enteral nutritional emulsion( TPF-D) by ICP-AES

目的：建立肠内营养乳剂（TPF-D）中钙、钾、镁、钠4种元素含量测定的新方法。方法：采用电感耦合等离子体发射光谱法（ICP-AES）,供试品经盐酸溶解处理后测定,钙、钾、镁、钠的发射谱线分别为422.7、766.5、285.2、589.6 nm。结果：钙浓度在0.4~1.6μg·mL-1、钾浓度在1~4μg·mL-1、镁浓度在0.15~0.6μg·mL-1、钠浓度在0.6~2.4μg·mL-1的范围内,与发射强度均呈良好的线性关系,加样回收率分别为100.8%、98.6%、101.5%、98.4%,最低定量浓度分别为0.003、0.002、0.001、0.002μg·mL-1。经与原标准中的火焰原子吸收法比较,准确度和精密度均能满足实验的要求,4种元素的含量测定结果基本一致。结论：该ICP-AES方法经方法学验证可用于同类营养制剂中钙、钾、镁、钠含量的测定。

Objective： To develop a new method for determination of the contents of four elements,including calcium,potassium,magnesium and sodium in enteral nutritional emulsion（ TPF- D）. Methods： Inductively coupled plasmaatomic emission spectrometry（ ICP- AES） was adopted,after samples were dissolved by hydrochloric acid. The emission lines of Ca,K,Mg and Na were 422. 7 nm,766. 5 nm,285. 2 nm and 589. 6 nm,respectively.Results： In the concentration ranges of calcium 0. 4- 1. 6 μg·mL- 1,potassium 1-4 μg·mL- 1,magnesium 0. 15- 0. 6 μg·mL- 1and sodium 0. 6- 2. 4 μg·mL- 1,the emission intensity and the concentration all showed good linear relationship,with the recoveries of 100. 8%,98. 6%,101. 5% and 98. 4%,respectively. The limits of quantification were 0. 003,0. 002,0. 001 and 0. 002 μg·mL- 1,respectively. Compared with the flame atomic absorption method in the original standard,the accuracy and precision could meet the requirements of the experiment,and the determination results of the contents of the 4 elements were basically identical. Conclusion： The ICP- AES method can be used for the content determination of calcium,potassium,magnesium and sodium in similar nutrition preparation after methodology verification.