罗非鱼鱼鳞与珍珠贝外套膜胶原蛋白酶解液的钙螯合能力对比研究

Comparative study of calcium chelating ability of tilapia fish scales and pearl oyster mantle fluid collagenase solution

利用Ⅰ-型胶原蛋白多肽制备螯合钙的研究已有较多报道。为了探索水产Ⅴ型胶原蛋白肽的钙螯合能力,利用4种酶酶解罗非鱼鱼鳞胶原蛋白(Ⅰ-型胶原蛋白)与珍珠贝外套膜胶原蛋白(Ⅴ-型胶原蛋白),得到胶原蛋白多肽,对鱼鳞胶原蛋白肽与珍珠贝外套膜胶原蛋白肽的钙螯合率做对比。发现4种酶中,无论用哪种单独处理得到的珍珠贝外套膜胶原蛋白肽均比同种酶处理得到的鱼鳞胶原蛋白肽钙螯合率高,且木瓜蛋白酶处理的2种胶原蛋白肽钙螯合率最高,分别为28.2%(鱼鳞)和34.5%(外套膜)。另外,对上述2种胶原蛋白肽利用5000 u的超滤膜分别过滤后,进行钙螯合率对比。结果,相对分子质量小于5000 u的V型胶原蛋白肽具有更强的钙螯合能力。该研究为珍珠贝外套膜的开发和利用提供了新的理论依据。

Research on the use of type-Ⅰ collagen peptides chelated calcium preparation have been widely reported. To explore the calcium chelating ability of the type-Ⅴ collagen peptides from aquatic products, in this paper, four kinds of enzymatic used to prepare tilapia fish scale collgen peptides（Type-Ⅰ collagen） and pearl oyster mantle fluid collagen peptides（Type-Ⅴ collagen）, then, contrast of the calcium chelate ratio of both collagen peptides. The results found that in these four types of enzyme, no matter what kind of enzyme been used, the obtained pearl oyster mantle collagen peptides’ calcium chelating rates higher than the scale collagen peptides. And, the peptide gotten by using papain has the highest rate of calcium chelating, respectively 28.2%（scale collagen） and 34.5%（mantle collagen）. In addition, using 5000 u ultrafiltration membrane treatment the above-mentioned two peptides, and contrasting the calcium chelating of both components, we found the type-Ⅴ collagen which with molecular less than 5000 u has stronger calcium chelating ability. This study provides a new theoretical basis for the development and utilization of pearl oyster mantle.