整合素α2(ITGA2)-807C/T位点基因多态性与糖尿病肾病的相关性

A correlation research between the Integrin alpha 2(ITGA2)-807 C/T gene polymorphism and diabetic nephropathy

目的：探讨桂北地区汉族人群整合素α2（ITGA2）基因807C/T位点基因多态性与糖尿病肾病（DN）的关系及其对血脂、载脂蛋白水平的影响。方法 ：选择住院确诊的2型糖尿病患者206例,再根据DN的诊断标准,分为糖尿病非肾病组（T2DM）及DN组,另选90例健康体检者为对照组（NC）。运用常规检测方法检测三组实验组的血清血脂及载脂蛋白水平,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测三组的基因型及等位基因频率分布。结果：与对照组相比,DN组的血脂TC、TG、LDL-C值均升高（P〈0.05）,DN组ITGA2基因-807C/T基因型分布及等位基因频率均有统计学差异（P〈0.05）;CT＋TT基因型及T等位基因频率显著高于对照组（均P〈0.05）;携带T等位基因的个体患DN的相对风险是携带C等位基因的1.72倍（OR=1.72,95%CI=1.176~2.517）。而T2DM组与对照组、DN组相比,ITGA2基因-807C/T基因型分布及等位基因频率差异均无统计学意义（均P〉0.05）。结论：ITGA2-807C/T基因多态性与广西桂北地区汉族人群的DN发病具有一定的相关性,其中T等位基因可能是DN发病的遗传易感基因,T等位基因可促进血脂TC水平的高度表达而使DN的发病风险增加。

Objective To explore the relation between the Integrin alpha 2 （ITGA2）-807 C/T gene and diabetic nephropathy （DN） in Han nationality in northern part of Guangxi. Methods 206 patients with type-2 diabetes were selected from the hospital. According to the diagnosis of diabetic nephropathy （DN） standards, 206 patients with type-2 diabetes were divided into diabetes without diabetic nephropathy （T2DM） and DN group, and 90 healthy people were selected as normal control （NC） group. The plasma lipid and lipoprotein levels were measured by routine method.And the genotypes and allele frequencies distribution were assessed by Polymerase chain reaction-restriction fragment length Polymorphism （PCR-RFLP）. Results compared with the control group,plasma total cholesterol（TC）,triglyceride（TG） and low density lipoprotein-cholesterol （LDL-C） levels in DN group blood were significantly higher （P〈 0.05）. ITGA2 gene - 807 - c/T genotype distribution and allele frequency in DN group were statistically significant （P〈0.05）. CT＋TT genotypes and T allele frequency in DN group were significantly higher than control group （P〈0.05）.The relative risk for DN in subjects carrying T allele was 1.72 times of that in the C allele carriers（OR = 1.72, 95% CI = 1.72 - 2.517）. And compared with control group and the DN group, ITGA2 gene-807-c/T genotype distribution and allele frequency differences in T2DM group had no statistical significance （P 〉 0.05）. Conclusions The polymorphism of ITGA2-807C/T gene is associated with the development of DN in Han nationality in northern part of Guangxi. The T allele may be a genetic susceptibility genes for DN, which may promote the level of TC high expression and increase the risk of DN.