经动脉与经静脉注射肾上腺素对小鼠心肺复苏疗效的影响

Impact of intra-arterial versus intravenous administration of epinephrine on the efficacy of CPR in mice

目的:对比经动脉与经静脉给予肾上腺素对心肺复苏疗效的影响。方法:20只雄性昆明小鼠,经食道快速起搏心室诱发室颤、建立心脏骤停(CA)模型,起搏开始后4 min随机经颈动脉(IA-gro=10)或经颈静脉(IV-gro=10)注射肾上腺素0.02 mg/kg,立即开始胸外心脏按压及机械通气,10 min无效则放弃复苏。主要观察自主循环恢复(restoration of spontaneous circulation,ROSC)率及ROSC后小鼠生存时间。结果:心肺复苏时经颈动脉或经颈静脉注射肾上腺素,两组小鼠的ROSC率无显著差异(10/10 vs 8/10)。自主循环恢复时间及生存时间也无显著差异[51±13 s vs 62±24 s;8.5(6.0,17.0)h vs 6.5(2.8,21.3)h,P>0.05]。结论:经动脉与经静脉注射肾上腺素对小鼠心肺复苏疗效无明显影响。

Objective To compare the impact of intra-arterial versus intravenous administration of epinephr-ine on the efficacy CPR in mice. Methods Transoesophageal cardiac pacing was performed to induce cardiac arrest for 4 minitues in 20 Kunming male mice. The mice were then randomized to two groups （n = 10 in each group）, and received epinephrine of 0.02 mg/kg via either carotid artery （IA-gro） or jugular vein （IV-gro） injection. Chest compression and ventilation were performed; and the rate of restoration of spontaneous circulation （ROSC） and survival time were recorded. CPR was stopped if spontaneous circulation was not restored within 10 minutes. Results There was no significant difference in the rates of ROSC between IA-gro and IV-gro （10/10 vs. 8/10, P＆gt;0.05）, nor in the time of ROSC or survival time [51 ± 13 s vs. 62 ± 24 s; 8.5 （6.0, 17.0） h vs. 6.5 （2.8, 21.3） h, P ＆gt; 0.05]. Conclusions Neither intra-arterial nor intravenous administration of epinephrine has no obvious impact on the efficacy of CPR in mice.