摘要
旋转定位方法在生物组织图像扫描或细胞注射实验中有着重要的应用。文中针对生物显微镜中旋转定位功能的缺失,设计了一种紧凑型步进电机驱动的旋转工作台,提出了使用不等长十字标记对齐法来辅助定位。利用特征跟踪算法实时追踪目标,再结合倒置显微镜自身的X/Y载物台和基于图像的视觉伺服控制器,完成生物样品的定位。实验显示,定位后两十字标记角度偏差为0.462°,位置差在2~3μm,满足生物操作要求。
Rotational positioning method has important application in image scanning of biological tissue or cell injection experiments. For the lack of rotational positioning function in biological microscope, a compact rotational stage driven by a stepper motor was designed in this paper, and cross mark alignment method with unequal length was proposed to aid positioning. Feature tracking algorithm is utilized to track the target with real time.Combined with the X/Y stage of the inverted microscope and image-based visual servo controller, positioning the biological sample could be completed. The experiment shows that, after positioning the two cross marks′ angle deviation is 0.462° and the position difference is 2~3 μm, meeting with the biological operation requirements.
出处
《机械设计》
CSCD
北大核心
2014年第8期79-82,共4页
Journal of Machine Design
基金
江苏省杰出青年基金资助项目(BK2012005)
江苏省青蓝工程中青年学术带头人项目
国家高新技术863计划资助项目(2012AAO40507)
关键词
旋转定位
旋转工作台
特征跟踪
视觉伺服控制
rotational positioning
rotational stage
feature tracking
visual servo control
