富硒酵母对小鼠IL-2水平、空斑形成细胞溶血能力及肠道菌群的影响

Effects of selenium-enriched yeasl on plasma IL-2 contents, hemolytic ability of plaque forming cells and intestinal flora in mice

为研究富硒酵母对小鼠血浆IL-2水平、空斑形成细胞溶血能力及肠道菌群的影响,本试验选用60只清洁级KM小鼠(雌雄各半),随机分为对照组、亚硒酸钠组和富硒酵母组,每日分别灌胃蒸馏水、亚硒酸钠(2μg Se/mL)和富硒酵母(2μg Se/mL)各0.5 mL。试验期为28 d。结果表明,富硒酵母组、亚硒酸钠组小鼠血浆IL-2含量和空斑形成细胞溶血能力的OD413较对照组分别提高18.24%(P<0.01)、17.65%(P<0.05)和12.82%(P<0.01)、7.69%(P<0.05);而富硒酵母组、亚硒酸钠组和对照组小鼠肠道中葡萄球菌、肠球菌、肠杆菌、乳杆菌和双歧杆菌数量均无显著差异(P>0.05)。

The experiment was conducted to determine the effects of selenium-enriched yeast on plasma IL-2 contents,hemolytic ability of plaque forming cells and intestinal flora in mice.Sixty KM mice,female and male in half,were randomly divided into control,sodium selenite and selenium-enriched yeast groups.0.5 mL of water, sodium selenite （2 μg Se/mL）and selenium-enriched yeast（2 μg Se/mL）were respectively supplemented to the three groups in oral（ig）every day. Whole feeding experiment lasted for 28 days.The results showed that plsama IL-2 contents and 0D413 of hemolytic ability of plaque forming ceils of selenium-enriched yeast group and sodium selenite group were 18.24% （P 〈 0.01 ）, 17.65% （P 〈 0.05） and 12.82% （P 〈 0.01 ）,7.69% （P 〈 0.05） higher than those of control group, respectively. However, there were no significant differences in the amounts of staphylococci, enterococci, enterobacteriaceae,lactobacilli and bifidobacterium in intestine of mice among selenium-enriched yeast group, sodium selenite group and control gronp（P 〉 0.05）.