一起鼠间鼠疫的实验室检测结果分析

Analysis of laboratory test results of plague endemic among wild rodent Meriones unguiculatus

目的通过对银川市滨河新区红墩子长爪沙鼠鼠疫疫源地自毙鼠及其体蚤、样方捕获鼠进行鼠疫抗原、抗体检测及鼠疫菌的分离,分析鼠疫菌的快速检验法,为动物鼠疫疫区判定和流行病学分析提供可靠的实验室依据。方法采用鼠疫菌常规检测结合胶体金快速检测试纸和PCR基因检测方法,CIN培养基和耶尔森菌专用培养基培养鼠疫菌。结果胶体金抗原检测和PCR基因检测结果阳性材料进行鼠疫菌培养均可分离出鼠疫菌;CIN显色平板更易于纯化培养及结果观察,操作简便省时、省力。结论在基层单位推广胶体金试纸和PCR基因检测诊断技术,节省确诊时间。

Objective To investigate the natural mortality in Meriones unguiculatusin Riverfront district Hongdunzi of Yinchuan, China, antigen test, antibody test, and isolation ofYersinia pestiswere performed on the carcasses of the rodents,ectoparasites, and quadrat samples, so as to find a quick detection method forY. pestis, and to provide a reliable laboratory basis for determination of animal epidemic area and epidemiological analysis of Y. pestis. Methods Yersinia pestiswas detected with the routine detection methods, colloidal gold method, and PCR. CIN medium and Y. pestis special medium were used in culture ofY. pestis. Results Yersinia pestiswas detected by colloidal gold antigen detection, PCR, and medium culture, while CIN color plate was an easier way in culture ofY. pestisand in reading of results. It is also time- and labor-saving.Conclusion Colloidal gold method and PCR detection should be adopted among the underprivileged health care units to save resources in the diagnosis of plague.