咖啡因对CUMS抑郁模型小鼠行为的影响

The Effect of Caffeine on Mice Behavior in Chronic Unpredictable Mild Stress Model

目的探讨咖啡因对慢性不可预见轻度应激(Chronic unpredictable mild stress,CUMS)抑郁模型小鼠行为的影响。方法选用C57BL/6J小鼠48只,分为正常+生理盐水组、正常+30 mg/kg咖啡因组、模型+生理盐水组和模型+咖啡因组(分别为5、15和30 mg/kg)6个处理组。利用制作的CUMS抑郁模型,分别给予生理盐水作对照或用不同剂量的咖啡因进行干预,持续造模22 d,观察小鼠的体质量增长变化,用旷场实验及液体消耗实验测定小鼠行为改变。结果造模22 d后,与正常+生理盐水组小鼠相比,模型+生理盐水组小鼠的体质量增长、水平垂直运动、液体消耗均未达到显著差异(P>0.05)。与模型+生理盐水组小鼠相比,模型+咖啡因组(5 mg/kg和30 mg/kg)小鼠的糖水消耗量和糖水偏爱百分比显著增高(P<0.05)。结论实验表明CUMS抑郁模型用C57BL/6J小鼠较难成功建构;咖啡因对小鼠CUMS抑郁模型的行为改变有部分改善作用,并呈剂量相关性;同时证明咖啡因的行为改善作用不是通过兴奋大脑皮层所致。该实验结果提示咖啡因具有潜在的抗抑郁作用。

Objective To investigate the effect of caffeine on behavior of mice,which suffered from chronic unpredictable mild stress （CUMS）.Method 48 C57BL/6J mice were divided into NC ＋ NS group,NC ＋ caffeine 30 mg/ kg group,CUMS ＋ NS group,CUMS ＋ caffeine group （5,15,30 mg/ kg） six groups.By the CUMS animal model,six groups were given physiological saline as a control or the intervention of different doses of caffeine for continuous 22 days of modeling.The body weight gain,in open field test and fluid consumption changes in mice were observed.Result During 22 days of modeling,compared with the normal ＋ NS group,the body weight,fluid consumption,behavior indicators in model ＋ NS group were not statistically significant （P ＞ 0.05）.Compared with model ＋ NS group,in 5mg/kg 和 30mg/kg caffeine intervention groups the 1％ sucrose preference and sucrose consumption showed significant difference （P ＜ 0.05）.Conclusion It is difficult to make successfully CUMS model of depression on C57BL/6J mice.The partially protective effect of caffeine on CUMS mice is dose related,and is not caused by excitement role of caffeine on cerebral cortex.