凹土玉米芯垫料对KM小鼠安全性影响的研究

Study for Effect of the Palygorskite Corncob Bedding on the Safety of KM Mice

目的观察非专业加工制作的普通刨花垫料、玉米秸垫料和凹土玉米芯垫料对KM小鼠体质量、血液生化指标、脏器病理组织学变化等的影响。方法 KM小鼠80只,随机分为4组,每组20只,雌雄各半,分别使用普通刨花、玉米秸和凹土玉米芯垫料,对照组不使用垫料,在相同饲养环境下饲养4周后,观察相应指标。结果凹土玉米芯组、玉米秸组与普通刨花组比较,小鼠体质量无显著性差异,P>0.05;普通刨花组雄性小鼠在第3周时体质量明显减低,与凹土玉米芯组比较有显著性差异,P<0.05。与凹土玉米芯组比较,普通刨花垫料组雌性、雄性小鼠的乳酸脱氢酶含量均有显著性升高,乙醇脱氢酶有显著性降低。普通刨花组小鼠与凹土玉米芯组小鼠比较谷丙转氨酶含量显著升高,玉米秸垫料组小鼠与凹土玉米芯组小鼠比较谷丙转氨酶升高。各组KM小鼠大体观察及解剖观察均未见异常,镜下观察组织切片,肺、心脏、肝、脾、胰腺、肾、膀胱、睾丸、卵巢均未见有意义的病理改变。结论凹土玉米芯垫料对小鼠的体质量及生化指标的影响较小,是实验动物接触性长期使用比较理想的垫料。

Objective To investigate the effects of bedding materials consisted of wood shavings,corn and the palygorskite corncob on the body weight,the blood biochemical parameters,pathological changes of KM mice.Method The 80 KM mice were divided into four groups randomly,20 in every group,with half male and half female,using the above-mentioned bedding materials and no bedding materials,respectively.The body weight were Weighed weekly,the blood biochemical parameters,including ALT,TP,ALP,LDH,T-SOD,ADH,were measured.Four weeks later,the pathological tissue organs were observed.Result Comparing with animals used the palygorskite corncob bedding,the weight of the male and the female KM mice in poplar bedding group was reduced from the third week,P ＜ 0.05.Compared with the KM mice in the palygorskite corncob bedding group,LDH of KM mice in poplar bedding group was raised,significantly,P ＜ 0.05; Compared with the KM mice in the palygorskite corncob bedding group,ADH of KM mice in poplar bedding group was reduced,significantly,P ＜ 0.05 ; Compared with the KM mice in the palygorskite corncob bedding group,ALT of KM mice in poplar bedding and corn bedding groups were raised,significantly,P ＜ 0.05 ; Significant pathological changes were not observed in the examined organs.Conclusion The palygorskite corncob bedding material has little effect on body weight,the blood biochemical parameters in KM mice,and the palygorskite corncob bedding material can be safely used in KM mice breeding.