高效液相色谱梯度洗脱法同时测定三七总皂苷中人参皂苷Rb_1、人参皂苷Rg_1和三七皂苷R_1含量

Simultaneous Determination of Ginsenoside Rb_1,Ginsenoside Rg_1 and Notoginsenoside R_1 Contents in Panax Notoginseng Saponins by HPLC Gradient Elution

目的 采用高效液相色谱梯度洗脱法测定三七总皂苷中人参皂苷Rb1、人参皂苷Rg1和三七皂苷R1的含量。方法 色谱柱为C18柱（250 mm×4.6 mm,3.5μm）,流动相为乙腈-水、梯度洗脱,检测波长为203nm,流速1.0 mL/min,柱温为室温。结果 人参皂苷Rg1进样量线性范围是6.0~18μg（r=0.996 7）,平均回收率为98.79%,RSD=0.33%（n=6）;人参皂苷Rb1进样量线性范围是6.0~18μg（r=0.996 4）,平均回收率为98.64%,RSD=0.46%（n=6）;三七皂苷R1进样量线性范围是1.6~4.8μg（r=0.997 1）,平均回收率为98.84%,RSD=0.56%（n=6）。结论 该方法准确、灵敏度高,可用于三七总皂苷的质量控制。

Objective To establish a HPLC gradient elution method for the determination of ginsenoside Rb1,ginsenoside Rg1and noto- ginsenoside R1 in Panax notoginseng saponins. Methods The HPLC method was carried out on the C18 column （250 mm ×4.6 mm, 3.5 μm） with acetonitrile-water as the mobile phase. The detection wavelength was 203 nm and the flow rate was 1.0 mL/min. Results The linear range of the ginsenoside Rgt sample size was 6.0- 18 μg（r=0. 996 7）,the average recovery rate was 98.79%, RSD = 0. 33% （n = 6）. The linear range of ginsenoside Rb1 sample size was 6.0- 18 μg（ r = 0. 996 4）, the average recovery rate was 98.64%, RSD =0. 46% （n = 6）. The linear range of notoginsenoside R1 sample size was 1.6-4.8 μg（ r = 0. 997 1 ）,the average recovery rate was 98.84%, RSD = 0. 56% （n = 6）. Conclusion This method is accurate and highly sensitive,and can be used to control the quality of Panax notoginseng saponins.