生血宝合剂中二苯乙烯苷的含量测定

Determination of Content of Stibene Glucoside in Shengxuebao Mixture

目的 建立测定生血宝合剂中君药制何首乌的有效成分二苯乙烯苷（2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷）含量的高效液相色谱（HPLC）法,为生血宝合剂的质量控制提供依据。方法 Waters alliance高效液相色谱仪,色谱柱为Suntek Kromasil C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水（20∶80）,流速为1.0 mL/min,检测波长320 nm。结果 二苯乙烯苷在0.054 60~1.092 00μg范围内与峰面积积分值线性关系良好（R2=0.999 9）。结论 该方法灵敏度高、定量准确、方法简便、重现性好,能有效控制生血宝合剂的质量。

Objective To establish a high performance liquid chromatography（HPLC） method for determining the content of 2,3,5, 4＇-tetrahydroxy- stilbene- 2- O- β- D- glycoside as the effective ingredients of the monarch drug prepared radix polygoni muhiflori in Shengxuebao Mixture. Methods The Waters alliance high performance liquid chromatograph was adopted with the chromatographic column of the SUNTEK Kromasil C18（250 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-water （20：80）,the flow rate was 1.0 mL/min and the detected wavelength was 320 nm. Results The sample size of 2,3,5,4＇ -tetrahydroxy- stilbene- 2- O- β- D- glycoside in the range of 0. 054 60-1. 092 00 μg showed good linear relationship with the peak area integral value（r=0. 999 9）. Conclusion This method is highly sensitive, accurately quantitative, simple and convenient with good reproducibility,and can effectively control the quality of Shengxuebao Mixture.