丹波黑大豆GmbHLH30转录因子耐铝功能初步研究

Study on the Function of Transcription Factor GmbHLH30 on Aluminum Tolerance Preliminary in Tampa Black Soybean

铝毒是酸性土壤作物生长的主要限制因素。前期研究发现,铝胁迫下,耐铝型丹波黑大豆SSH(suppression subtractive hybridization,SSH)cDNA文库中bHLH30转录因子基因上调表达,推测该基因与丹波黑大豆耐铝性相关。克隆GmbHLH30基因,构建GmbHLH30植物表达载体pK2-35S-GmbHLH30,并在烟草中过量表达获得转GmbHLH30的转基因烟草植株。在铝胁迫下,转GmbHLH30的转基因烟草相对根伸长率比野生型烟草大,可溶性糖和脯氨酸含量高,H2O2水平低。表明GmbHLH30基因的过量表达可以增强植物的耐铝能力,暗示GmbHLH30转录因子参与调控植物的耐铝特性。

Aluminum toxicity is the major limiting factor of crop growth in acidic soils. Previous studies found that the expression of bHLH 30 transcription factor gene is upregulated in SSH（suppression subtractive hybridization, SSH） cDNA library of resistant aluminum type Tamba black soybean under Al stress. It was speculated that the gene was associated with aluminum tolerance of Tamba black soybean. GmbHLH30 gene was cloned and plant expression vector pK2-35S-GmbHLH30 was constructed, the vector was overexpressed in the tobacco. The GmbHLH30 transgenic tobacco lines have bigger relative root elongation rate,higher soluble sugar and proline as well as lower H2O2 content in leaf and root, compared with wild-type tobacco under aluminum stress. These results indicate that GmbHLH30 transcription factor genes would improve the ability of plants resistant aluminum and suggested that GmbHLH30 transcription factor genes participate in adjusting aluminum resistant characteristic of plant.