定量研究石斛醇提取物对酵母朊病毒的治愈作用

Quantitative Research of the Curing Effects of Alcohol Extract from Dendrobium on Yeast Prion [PSI^+]

为研究石斛醇提取物的抗朊病毒作用,借助酵母朊病毒[PSI+]表型系统分析石斛醇提取物对酵母朊病毒[PSI+]表型的作用,引入半变性琼脂糖凝胶电泳技术在蛋白质水平分析石斛醇提取物对酵母朊病毒的治疗效果。结果显示,石斛醇提取物浓度在15 g/L时,作用酵母朊病毒[PSI+]细胞5 d的治愈率为5%;并且石斛醇提取物浓度在5~20 g/L范围内时,药物剂量与酵母朊病毒[PSI+]表型治疗效果呈现较好正相关性。蛋白水平试验表明石斛醇提取物作用酵母朊病毒[PSI+]细胞5 d后红色菌落的朊病毒聚集体大小与[psi-]相似。

In order to study the anti-prion effects of alcohol extract from dendrobium, the phenotype system of yeast prion [PSI^＋] was used to assay effects of dendrobium on [PSI^＋], and the semi-denaturing agarose gel electrophoresis technique was introduced to detect the effects of dendrobium on yeast prion at the protein level. The results showed that the curing rate of [PSI^＋] cells were 5% after 5 days treatment with dendrobium at the concentration of 15 g/L; and the concentration of dendrobium had a positive relationship to its curing effects on yeast prion [PSI^＋] phenotype at the concentration rage of 5-20 g/L. Protein experiments showed that the aggregation status of red phenotype cells in 5 days dendrobium treated cells were similar to those in[psi^-] cells.