重症急性胰腺炎大鼠模型制备方法的改进

The improvement of severe acute pancreatitis rat model preparation methods

目的探讨3.5%牛磺胆酸钠胰腺被膜下均匀多点注射诱导重症急性胰腺炎(SAP)大鼠模型方法的改进。方法96只健康SD大鼠随机分成SAP组(n=42)、假手术组(n=42)和空白对照组(n=12)。SAP组在胰腺被膜下均匀多点注射3.5%牛磺胆酸钠,假手术组同法注射0.9%生理盐水,两组均于术后2、4、6、12、24、48h分别处死7只大鼠测血清淀粉酶值,观察胰腺大体形态及光学显微镜下的病理改变。空白对照组大鼠于实验前全部处死,测得正常血清淀粉酶值并制作正常胰腺组织标本作为参考对照。结果 SAP组各时间点血清淀粉酶值均较假手术组、空白对照组对应时间点明显升高(P<0.000 1),假手术组、空白对照组之间比较差异无统计学意义(P>0.05)。SAP组胰腺大体组织及光学显微镜下病理改变均较假手术组、空白对照组明显。结论改良的胰腺被膜下注射法能制备典型的SAP模型,且具有操作简单、诱导成功率高、结果稳定和病死率低等优点,是一种较为理想的制模方法。

Objective To study severe acute pancreatitis（SAP）in rats model method improvement,these rates were given uniform and multi-point injecting with 3.5%of the taurocholic acid sodium in pancreas capsule.Methods 96 healthy SD rats randomly divided into SAP group（n=42）and sham-operated group（n=42）,blank control group（n=12）.SAP group was given uniform multi-point injection of 3.5%sodium taurocholic acid in the pancreas capsule,Sham-operated group was injected with 0.9%saline injection,two groups were recorded the measurement of serum amylase values by killing 7rats in 2,4,6,12,24 and 48hrespectively,and observed pancreas in form and then pathological changes.The whole rates of control group were killed before the experiment to measure normal values of serum amylase and make normal pancreas tissue specimen as a reference.Results Each time point of the serum amylase values of SAP group were obviously increased compared with the corresponding time points sham-operated group and blank control group（P〈0.000 1）,no difference between sham-operated group and blank control group;Pancreas gross tissue and then observed pathological changes of SAP group are more obvious than the sham-operated group and blank control group＇s.Conclusion The improved injection in the pancreas capsule is an ideal method of moulding,which can make typical SAP model and have simple operation,induced by a high success rate,and have the stability and low mortality rate.