积雪草颗粒对糖尿病肾病大鼠肾损伤的影响

Effect of Centella Asiatica on Renal Injury of Diabetic Rats

目的:探讨积雪草颗粒(CTA)减轻糖尿病肾病(DN)大鼠肾损伤的效果及可能机制。方法:45只12周龄清洁级SD雄性大鼠,随机分为正常对照组(NC组),糖尿病组(DN组),糖尿病治疗组(DT组),共治疗12周。12周后测定24小时尿微量白蛋白,心脏采血检测血糖(BG)、血肌酐(Scr)、糖化血红蛋白(HbA1c);分离双肾,检测肾皮质中氧化产物丙二醛(MDA)、抗氧化指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平。采用RT-PCR法检测各组大鼠肾皮质中GSH-Px、Cu-Zn SOD的mRNA表达。结果:与NC组比较,DN组大鼠BG、Scr较NC组均升高(P<0.05);DT组Scr较DN组下降(P<0.05)。与NC组比较,DN组MDA水平显著增高,SOD、GSH-Px水平降低;肌酐清除率显著降低(P<0.05);与DN组比较,DT组MDA水平显著降低,SOD、GSH-Px水平较高(P<0.05),差异有统计学意义(P<0.05)。与NC组比较,DN组SOD、GSH-Px RNA表达水平显著增高;与DN组比较,DT组SOD、GSH-Px RNA水平明显增高(P<0.05),差异有统计学意义(P<0.05)。结论:CTA对糖尿病肾病大鼠有保护作用,其作用机制与抑制肾脏氧化应激有关。

Objective：to investigate the Centella asiatica（CTA） protective effects on rats with diabetic nephropathy （DN） and possible mechanisms. Methods：Forty-five 12-week mature male SD rats of clean degree, were randomly divided into normal control group （group NC）, diabetic group（ group DN）, diabetic treatment group（ group DT）, treatment for 12 weeks. After 12 weeks, To detect 24 hours uri- nary albumin, Then all rats were killed, collected the heart blood to detect blood glucose （BG）, serum creatinine （Scr）, glycosylated he- moglobin（ HbAI c）. To separate double kidney, detect the oxidation product malondialdehyde （MDA） level in renal cortex, antioxidant index of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） level. To detect the expression of GSH-Px, Cu-Zn SOD mR- NA in renal cortex of rats by RT-PCR in every group. Results：Compared with NC group, Scr,BG lecvel in DN group significantly in- creased （ P 〈 0.05 ） ; while, Scr level of DT group was lower than that of group DN （ P 〈 0.05 ）. Compared with NC group, the MDA level of DN group significantly increased, and SOD, GSH-Px levels decreased ;creatinine clearance decreased significantly（ P 〈 0.05 ）. In DT group, MDA level were significantly lower, the level of SOD ,GSH-Px was higher than that in DN group（ P 〈 0.05 ）. The difference was statistically significant. Compared with NC group, the expression level of SOD ,GSH-Px RNA of DN group was significantly higher;com- pared with DN group, the expression level of SOD, GSH-Px RNA in group DT was significantly higher（ P 〈 0.05 ） , The difference was statistically significant. Conclusions：CTA has protective effects on rats with diabetic nephropathy and its mechanism is associated with the inhibition of oxidative stress in the kidney.