苦参碱对人胚肺成纤维细胞增殖和凋亡的干预作用

Intervention Effect of Matrine on Proliferation and Apoptosis of Human Embryonic Lung Fibroblast

目的:观察苦参碱对人胚肺成纤维细胞增殖和凋亡的影响。方法:选择苦参碱为干预药物,以不同浓度梯度作用于体外培养的人胚肺成纤维细胞,采用MTT法检测细胞增殖,用流式细胞仪分析细胞周期和凋亡率。结果:MTT结果显示,各干预组对HFL-1细胞生长均有显著抑制增殖作用,其强度呈时间-剂量依赖性。苦参碱作用于HFL-1细胞48 h后,各干预组细胞总凋亡率均明显高于对照组(P<0.05);与对照组比较,苦参碱各浓度组HFL-1细胞G0/G1期百分率显著上升(P<0.05)。结论:苦参碱可在一定程度上抑制人胚肺成纤维细胞增殖,并诱导其凋亡。

Objective：To observe the effect of matrine on prnliferation and apoptosis of human embryonic lung fibroblast. Methods：The human embryonic lung fibroblasts with matrine of different concentration were cultivated, cell prolifertion was measured by thiazolyl blue （MTT） assay;Cell cycle and apoptosis rate were determined with flow cytometre. Restults：MTT results showed that each intervention group has significantly inhibition effect on cell growth of HFL-I ,in dose-dependent and time-dependent manner. When treated with ma- trine of different concentration after 48 hours, the total apoptosis rate were significantly higher than control group（ P 〈 0.05 ） ;compared with the control group,HFL-1 cells of marine concentration groups in G0/G1 phase percentage rise significantly（ P 〈 0.05 ）. Conclusion：Matrine can inhibit the proliferation of HLF-1 to a certain extent,and induce its apoptosis.