合并心肌损伤的窒息新生儿血浆心肌营养素-1及糖原磷酸化酶同工酶脑型的测定及临床意义

Detection and Clinical Significance of Plasma Cardiotrophin-1 and Glycogen Phosphorylase Isoenzyme BB in Asphyxiated Newborn Infants with Myocardial Injury

目的探讨合并心肌损伤的窒息新生儿血浆心肌营养素-1(cardio trophin-1,CT-1)以及糖原磷酸化酶同工酶脑型(glycogen phosphorylase isoenzyme BB,GPBB)的变化及其临床意义。方法选择72例合并心肌损伤窒息新生儿为试验组[其中轻度窒息39例(轻度窒息组),重度窒息33例(重度窒息组)],32例健康新生儿为对照组,采用酶联免疫吸附法(ELISA)测定血浆GPBB、CT-1水平,并分析血浆GPBB、CT-1水平与窒息程度的关系。结果试验组血浆GPBB、CT-1水平均明显高于对照组(均P<O.05);重度窒息组血浆GPBB、CT-1明显高于轻度窒息组(均P<O.05);血浆GPBB水平、CT-1与窒息程度均呈负相关(r=-0.519、r=-0.527,P<O.05);血浆GPBB水平与CT-1水平呈正相关关系(r=0.547,P<O.05)。结论合并心肌损伤的窒息新生儿血浆CT-1以及GPBB水平明显升高,且与窒息程度密切相关,窒息程度越重其水平越高,提示与新生儿窒息心肌损伤有密切关系;测定血浆CT-1以及GPBB水平有助于判断新生儿窒息合并心肌损伤的程度。

Objective To observe the changes in plasma cardiotrophin-1 （CT-1）and glycogen phosphorylase isoenzyme BB （GPBB） and their clinical significance in asphyxiated newborn infants with myocardial injury. Methods The levels of plasma CT-1 and GPBB were detected by ELISA in 72 asphyxiated newborn infants with myocardial injury （experimental group） and 32 healthy newborns （control group）.In addition, infants in experimental group were further divided into mild asphyxia group （n=39）and severe asphyxia group （n=33）. The relationships of asphyxia degree to CT-1 and GPBB levels were analyzed. Results Compared with control group, levels of GPBB and CT-1 significantly decreased in experimental group（P〈0.05）.Compared with mild asphyxia group,levels of GPBB and CT-1 significantly increased in severe asphyxia group. The levels of plasma GPBB and CT-1 were negatively correlated with the degree of asphyxia （r=-0.519 and r=-0.527, respectively, P〈0.05）. Moreover, GPBB levels were positively correlated with CT-1 levels （r=0.547, P〈0.05）. Conclusion The levels of plasma GPBB and CT-1 increase obviously and are negatively correlated with the degree of asphyxia in asphyxiated newborn infants with myocardial injury, suggesting that there is a relationship between neonatal asphyxia and myocardial injury. The detection of plasma GPBB and CT-1 levels can help to determine the degree of neonatal asphyxia with myocardial injury.