在线微透析-液相色谱-串联质谱法测定人参水提物对糖尿病脑病大鼠脑内神经活性物质的影响

Effects of Ginseng Aqueous Extract on Neurochemicals in Diabetic Encephalopathy Rat Brain by Online Microdialysis-LC-MS /MS

评价人参水提物对糖尿病脑病大鼠学习记忆能力及脑内神经活性物质的影响。建立糖尿病模型,采用Morris水迷宫实验以逃避潜伏期(ELT)、穿越目标区域次数及中心区域(%)为指标评价大鼠的学习记忆能力。利用在线微透析-液相色谱-串联质谱法,Venusil C18柱梯度洗脱,MRM方式检测各组大鼠海马区8种神经活性物质水平并进行比较。结果表明:糖尿病大鼠人参治疗后认知能力明显改善(p<0.05);在线检测方法线性良好(R2>0.99),准确度和精密度满足分析要求;相比模型组,人参组大鼠脑内牛磺酸、乙酰胆碱水平显著升高(p<0.01),谷氨酸、丝氨酸、天门冬氨酸、γ-氨基丁酸、多巴胺和五羟色胺水平均显著降低,8种神经活性物质水平均向正常水平调节。

To evaluate the effects of ginseng aqueous extract on learning and memory abilities and neurochemicals in diabetic encephalopathy rat brain. Diabetes mellitus model was established. Cognitive abilities were evaluated with Morris water maze test by the indexes of escape latency time （ ELT） , times of passing through the target area and the percent of swimming track in goal quadrant. We applied online MD-LC-MS/MS method to determine eight neurochemicals in rat hippocampus among different groups in MRM mode. Venusil C18 column was used for separation. Treatment with ginseng aqueous extract significantly improved the cognitive abilities of diabetic rats （p〈0. 05）. The method had excellent linearity （R2〉0. 99）. The accuracy and precision could meet the analysis requirement. Comparing with the model group, the concentrations of taurine and acetylcholine were significantly increased （ p〈0 . 01 ） after treated with ginseng aqueous extract, but the glutamic acid, aspartic acid,γ-aminobutyric acid, serine, dopamine and serotonine decreased dramatically. Ginseng aqueous extract can efficiently regulate the levels of eight neurochemials in diabetic rat brain to normal.