乳癖康胶囊指纹图谱实验条件探索

Exploration on Experimental Conditions of Fingerprint in Rupikang Capsules

目的:探索乳癖康胶囊指纹图谱的实验条件。方法:采用高效液相色谱法,色谱柱为Agilent ZORBAX SB-C18柱,(4.6 mm×250 mm,5μm),对样品的提取溶剂、提取方法、检测波长、洗脱溶剂、洗脱方法进行了选择。结果:本试验采用80%甲醇为提取溶剂,超声60 min提取,选择280 nm作为检测波长,采用洗脱条件4,进行乳癖康胶囊HPLC指纹图谱研究较好。结论:实验证明,该文选定的色谱方法可操作性强,重现性好,可作为乳癖康胶囊指纹图谱研究的基础。

Objective ： To explore the experimental conditions of fingerprint in Rupikang Capsules. Methods ： HPLC analysis was carried out on Agilent ZO1RBAX SB-CI8 （ 4.6 mm × 250 mm,5 μm ）. The sample experimental conditions of the extraction solvent and extraction method were selected and wavelengthan and elution solvent and clution method were determined. Results： 80% ethanol was as the extraction solvent, ultrasonic extraction 60 min, wavelengthan 280 nm, selecting elution condition 4. Conclusion ： The method is easy to operate and can be used for the foundation of the fingerprint study of Rupikang Capsules.