芯片技术对南昌地区HIV-HCV合并感染者的HCV基因型研究

The analysis of HCV genotype with the DNA-chip technology in the concurrent infection of HIV-HCV patients in Nanchang

目的了解芯片技术对南昌地区HIV-HCV合并感染者的HCV基因型分布。方法 2008年10月至2013年11月期间,在南昌地区就诊的285例HIV感染患者进行HCV筛查,并对HCV阳性患者的基因型进行芯片技术检测,将其检测结果与测序结果进行比对;同时对HIV-HCV合并感染者进行流行病学调查。结果芯片技术可检测出约900拷贝/ml的丙型肝炎病毒的基因型,HIV感染者中,HCV阳性比率为19.3%,HIV-HCV合并感染者的HCV基因型分布情况为1b型41例,2a型9例;3b型1例,6型1例,还有1 b+2a型3例;芯片检测结果与基因测序结果完全一致。结论芯片技术灵敏度高,准确性好,HCV基因型分布以1b基因型为主,其次为2a型;HIV感染者的感染传播途径有了新变化,性传播感染HIV比例在增加,但性传播合并感染的比例较低。

Objective To evaluate the distribution of HCV genotype with the DNA-chip technology in the concurrent infec-tion of HIV-HCV patients in Nanchang. Methods During October 2008 and Novembe 2013, HCV screening were performed in 285 HIV patients, the HCV genotype was detected by DNA-chip technology, and the results were compared with the DNA se-quencing results. Results DNA-chip technology could detect about 900 copies/ml of HCV DNA. The positive rate of HCV infec-tion was 19.3%in HIV patients. 41 patients had the 1b HCV genotype, 9 patients had the 2a HCV genotype, 1 patient had the 3b HCV genotype, 1 patient had the 6 HCV genotype, 3 patients had the 1 b＋2a HCV genotype.The results of the DNA-chip technol-ogy and the results of DNA sequencing were the same. Conclusion The DNA-chip technology has high sensitivity and accuracy. The major HCV genotype in HIV patients is 1b, and then 2a. The HIV transmission route has changed, the sexually transmitted HIV is increasing, but the rate of concurrent infection is low.