膜性肾病差异性表达microRNA的靶基因功能分析

Functional analysis of target gene based on membranous nephropathy different expression microRNA

目的：探讨膜性肾病（MN）差异性表达microRNA的靶基因主要参与的Gene Ontology（GO）富集与Kyo-to Encyclopedia Genes And Genomes（KEGG）通路．方法：100例MN患者与100例健康对照者（NC）作为实验对象．100例NC随机分成10组（NC1～10），每组10人．同样100例MN随机分成10组，每组10人（MN1～10）．分别从实验参与者静脉采取全血10 mL，来源于静脉血的单个核细胞用于提取RNA．总RNA以组为单位等量混合用于高通量技术测序（high-throughput sequencing），对RNA测序结果进行长度分布，基因比对，RNA分类注释，RNA特有序列及其公共序列分析后，获得小核糖核酸（microRNA）进行MN与NC差异性表达分析来寻找显著表达microRNA．应用相关的软件对差异性表达的microRNA进行靶基因预测，靶基因借助功能注释软件对其参与的生物过程进行GO富集及KE GG通路分析．结果：靶基因在GO富集分析中主要集中在细胞器官组成，细胞膜组成，离子结合，生物代谢过程，生物调节过程等．靶基因在KE GG通路分析中主要参与嘌呤代谢通路，代谢产物生物合成，癌症通路，蛋白激酶信号通路等．结论：MN与NC之间存在着显著差异性表达microRNA．差异性表达的microRNA的靶基因参与的GO富集与KE GG通路可能与MN的发病机制与临床症状有着密切的联系．

Aim：To explore the target gene which came from different expression microRNA in mem-branous nephropathy （MN）and mainly participated in the Gene Ontology （GO）enrichment and Kyoto Encyclopedia Of Genes And Genomes （KEGG）pathways.Methods：The participants in this research included 100 MN patients and 100 healthy controls （NC）.One hundred healthy controls were divided in-to 10 groups,each group contained 10 persons.And 100 MN were also divided into 10 groups,each group contained 10 persons.Draw venous blood （10 mL）from each participant,respectively.Then the peripheral blood mononuclear cells were separated from venous blood to extract total RNA.Equal total RNA was compounded base on groups as unit was subjected to high-throughput sequencing.After this primary process,the RNA was used to preliminary analysis which included length distribution,genome mapping,RNA annotation,common and specific sequences accounted.The microRNA finally was ob-tained.We made a comparsion of microRNAs between MN and NC groups to find the different expression microRNA.The different expression microRNAs were used to predict target microRNA through relevant software.The target genes came from different expression microRNAs were subjected to GO enrichment and KEGG pathways analysis.Results：The target genes mainly enriched in cellular part,cellular mem-brance,ion binding,biopolymer metabolic process,regulation of biological process.For the KEGG path-way,the target gene mainly participated in purine metabolism,biosynthesis of secondary metabolites, pathway in cancer,MAPK signaling pathway.Conclusion：There were different expression microRNAs between MN and NC1 ~10 groups.The target gene came from different expression microRNAs that in the GO enrichment and KEGG pathway analysis could have the relation with the pathogenesis and clinical symptom of MN.