香蕉愈伤组织诱导培养中褐化抑制剂的筛选

Screening of Anti-browning Agents in Banana Callus-inducing Culture

为解决香蕉愈伤组织诱导培养过程中的外植体的褐化现象,分别用AgNO3(10,20 mg·L-1),AC(1,2,3 g·L-1),PVP(1,2 g·L-1),VitC(100,200 mg·L-1)等防褐化剂,对香蕉的愈伤组织诱导进行防褐化研究。结果表明:外植体培养10 d后,所有防褐化处理实验组的褐化率均低于对照组,说明在愈伤组织诱导的早期,不同的防褐化剂均有一定的防褐化效果;随着培养时间延长,不同的防褐化处理逐渐出现差异,在愈伤组织诱导培养40 d后,活性炭的防褐化效果最好,其中1 g·L-1活性炭的褐化率仅为20%,效果最佳。

Anti-browning agents,AgNO3（10,20 mg· L-1）,activated carbon （1,2,3 g· L-1）,Polyvinyl Pyrrolidone （PVP） （1,2 g · L-1）,Vitamin C （100,200 mg · L-1） were supplemented in the banana culture medium to inhibit explant browning of banana during the callus inducing process.Ten days after cultured the explants treated with anti-browning agents showed a lower browning rate than those in the control.This indicated that all the anti-browning agents were effective in inhibiting explant browning at the early stage of callus inducing culture.However,with the time of tissue culture going on,the anti-browning agents tended to be significantly different in inhibition of explant browning,and 40 days after callus-inducing culture the activated carbon treatments showed the highest inhibition of explant browning,of which the 1 g · L-1 activated carbon was optimum with a browning rate of only 20％ of the banana explants.