阿托伐他汀对局灶性脑缺血大鼠脑组织超氧化物歧化酶的影响

Effect of atorvastatin on brain tissue superoxide dismutase of focal cerebral ischemia rats

目的探讨阿托伐他汀对局灶性脑缺血大鼠脑组织超氧化物歧化酶(SOD)活性的影响。方法选用健康Sprague-Dawley(SD)大鼠,随机分为阿托伐他汀预处理组、单纯缺血组和假手术组。阿托伐他汀预处理组于术前15 d灌胃阿托伐他汀,6 mg/(kg·d),然后制备大鼠局灶性脑缺血模型,缺血后24 h行神经行为学评分、化学比色法测定脑组织匀浆中SOD活性。结果与假手术组比较,局灶性脑缺血24 h,脑组织SOD活性明显下降(P<0.05)。与单纯缺血组比较,阿托伐他汀预处理组明显降低神经行为学评分(P<0.05),提高脑组织匀浆SOD活性(P<0.05)。结论阿托伐他汀可以改善局灶性脑缺血大鼠神经行为学评分,提高脑组织SOD活性,增强氧自由基清除作用,具有神经保护作用。

Objective To explore the effect of atorvastatin calcium on brain tissue superoxide dismutase（SOD） of focal cerebral ischemia rats. Methods Healthy Sprague-Dawley （SD） rats were randomly divided into atorvastatin pretreatment group, pure ischemia group and sham-operated group. Atorvastatin pretreatment group was treated by atorvastatin calcium at a dose of 6 mg/（kg·d） 15 days before the operation. made the focal cerebral ischemia model. 24 h after ischemia, neurobehavioral scores and chemical colorimetric method were used to detect SOD activity in brain tissue homogenate. Results Compared with sham-operated group, 24 h after focal cerebral ischemia, SOD activity declined. Compared with pure ischemia group, the neurobehavioral scores declined（P〈0.05）, SOD activity in brain tissue homogenate in atorvastatin pretreatment group improved（P〈0.05）. Conclusion Atorvastatin can improve neurobehavioral scores in the focal cerebral ischemia rats, increase SOD activity in brain tissue and scavenging effects of oxygen free radical atorvastatin has neuroprotective effect against focal cerebral ischemia in rats.