铜、维生素A及其交互作用对肉仔鸡肝脏抗氧化酶活性的影响

Effect of Copper,Vitamin A and Its Interaction on Antioxidant Enzyme Activity in Liver of Broilers

选用1日龄艾维茵肉仔鸡448只,采用4×2完全随机试验设计,研究日粮添加不同水平的Cu和VA对肉仔鸡不同生长阶段（前期0-4周龄和后期5-7周龄）肝脏内抗氧化酶活性的影响。结果表明：1高Cu组（225mg/kg）对前期肝脏总超氧化物歧化酶（T-SOD）活力的影响极显著（P≤0.01）;低Cu组对全期肝脏总抗氧化能力（T-AOC）影响显著（P≤0.05）;低Cu组对肝脏铜蓝蛋白（Cp）活力最高,对全期肝脏Cp活力影响极显著（P〈0.01）。25 000 IU/kgVA对前期肝脏T-SOD活力影响极显著（P〈0.01）;1 500 IU/kg VA对全期肝脏一氧化氮合成酶（NOS）和肝脏谷胱甘肽过氧化物酶（GSH-Px）活力影响极显著（P≤0.01）;对全期肝脏T-AOC影响显著（P〈0.05）;对后期肝脏Cp活力影响极显著（P〈0.01）。3铜与VA交互作用对前后期肝脏T-SOD活力影响均显著（P〈0.05）;对后期肝脏锌铜超氧化物歧化酶（CuZn-SOD）和肝脏T-AOC活力影响显著（P〈0.05）;对前后期肝脏NOS活力影响均不显著（P〉0.05）;对前后期肝脏GSH-Px和肝脏Cp活力影响极显著（P〈0.01）。由此可见,铜与VA交互作用能显著影响前后期肝脏T-SOD、GSH-Px和Cp活力及后期肝脏CuZn-SOD与T-AOC活力。

A 4×2 completely random experiment was designed to investigate the influence of different Cu andvitamin A（VA）levels of dietary on antioxidant enzyme activity in broiler′s liver at 0 to 4 weeks of age and 5 to 7 weeksof age with 448 1-day-age AVIAN broilers. The results showed that：1dietary 225 mg/kg copper had a significanteffect on total superoxide dismutase（T-SOD）activity in liver from 0 to 4 weeks of age（P≤0.01）;the effect of lower copper group on total antioxidant capacity（T-AOC）was highest,there was a significant effect on ceruloplasmin（Cp）activity in liver during the whole period（P〈0.01）;2dietary 5 000 IU/kg VAhad a significant effect on T-SOD activityin liver from 0 to 4 weeks of age（P〈0.01）;dietary 1 500 IU/kg VAcould significantly affect nitric oxide synthase（NOS）and glutathione perosidase（GSH-Px）activity in liver during the whole period（P≤0.01）;dietary VAhad asignificant effect on T-AOC activity in liver during the whole period（P〈0.05）,could significantly affect Cp activity inliver from 5 to 7 weeks of age（P〈0.01）;3the interaction between copper and VAcould significantly affect T-SODactivity in liver during the whole period（P〈0.05）,had an extremely significant effect on CuZn-SOD and T-AOC activityin liver from 5 to 7 weeks of age（P〈0.05）,couldn′t significantly affect NOS activity in liver during the whole period（P〉0.05）,could significantly influence GSH-Px and Cp activity in liver during the whole period（P〈0.01）. Therefore,interaction of copper and VAhad a significant effect on T-SOD,GSH-Px and Cp activity in liver during the wholeperiod,and could significantly influence CuZn-SOD and T-AOC in liver from 5 to 7 weeks of age.