猪瘟病毒N^(pro)蛋白与宿主蛋白β-actin相互作用的鉴定

Identification of the interaction between classical swine fever virus N^(pro) protein and porcine β-actin

为研究与猪瘟病毒(CSFV)Npro蛋白相互作用的宿主蛋白,本研究采用酵母双杂交方法从猪外周血单个核细胞cDNA表达文库中筛选获得与Npro蛋白相互作用的宿主蛋白β-actin,经共转化试验和免疫共沉淀试验表明两者存在特异的相互作用,激光共聚焦试验显示两者共定位于细胞浆。本研究表明Npro蛋白与宿主细胞β-actin蛋白存在相互作用的关系。

To study the host cellular proteins interacting with the classical swine fever virus （CSFV） Nproprotein, yeast two-hybrid assay was used to screen from the swine peripheral blood mononuclear cells （PBMC） cDNA expression library. β-Actin was identified as a potential interacting partner of Npro protein. Co-transformation and co-immunoprecipitation （co-IP） analysis also confirmed the interaction between Npro and β-actin. Laser confocal assay showed thatNpro and β-actin colocalized in cytoplasm. These results demonstrated that CSFV Npro interacted with β-actin.