内皮脂肪酶584C／T基因多态性与高血压病相关性研究——299例常州地区汉族人群分析

Association of endothelial lipase 584C/T polymorphism with essential hypertension： analysis of 299 Han population from Changzhou region

目的探讨内皮脂肪酶（EL）584C／T基因多态性与高血压病（EH）的相关性。方法本研究共纳入160名EH患者和139名对照者。EL584C／T基因型检测采用聚合酶链反应一限制性内切酶片段多态性（PCR．RFLP）方法检测。结果EH组CC、CT、TT基因型频率分别为56．3％，42．5％，1．1％；对照组CC、CT、TT基因型频率分别为56．1％，38．8％，5．0％。T等位基因频率在两组间分别为22．5％和24．5％。两组间基因型频率和等位基因频率比较，差异均无统计学意义。Logistic回归显示在矫正传统的EH高危因素后，EL584C／T多态性仍然与EH之间无相关性。本研究同时表明EL584C／T多态性与血脂之间无明显相关性。结论EL584C／T基因多态性与常州地区汉族人群EH之间无明显相关性。

Objective To investigate the association of endothelial lipase （EL） 584C/T polymorphism with essential hypertension （EH）. Methods The EL 584C/T genotypes were detected using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis in 160 EH patients and 139 sex- and age-matched controls living in Changzhou region. Results The frequencies of the CC, CT and TT genotypes were respectively 56.3%, 42.5% and 1.1% in EH group, and 56.1%, 38.8% and 5.0% in control group, respectively. The frequency of T allele was 22.5% and 24.5% in EH and control groups, respectively. There was no significant difference between EH and control groups in genotypic and allelic frequencies. Logistic regression analysis showed that there was no significant correlation between the EL 584C/T gene polymorphism and EH after adjustment of traditional EH risk factors. There was no significant correlation between EL 584C/T genotypes and blood lipids levels. Conclusion There is no significant correlation of EL 584C/T polymorphism with Han nationality EH patients in Changzhou region.