EZH2蛋白在人胎儿器官发育过程中的表达模式

Expression pattern of EZH2 in oganogenesis of human fetuses

目的:检测果蝇zeste基因增强子人类同源物2(EZH2)蛋白在人胎儿发育过程中的表达模式,为了解EZH2在胎儿发育过程中可能的作用,提供新的、多器官的信息。方法:收集不同胎龄流产或死产胎儿33例及1例新生儿标本。选取食管、胃、小肠、结肠、肝、胰、腮腺、甲状腺、肾上腺、肺、气管、心脏、大血管(主动脉或肺动脉)、肾、膀胱、子宫、卵巢、睾丸、脾、胸腺、大脑等器官组织,经HE染色辨别形态学结构并构建组织芯片后,进行EZH2免疫组化染色检测。结果:在所有检测的上述胎儿器官中EZH2蛋白均有表达,但在不同的组织器官和不同的胎龄其表达水平不同。EZH2蛋白阳性染色主要定位于细胞核,在部分细胞同时也有细胞质阳性染色。结论:EZH2在人类胎儿期的各器官组织均有表达,表达模式有胎儿发育时间的差异性和高度的组织特异性。

To determine the expression pattern of EZH2 protein in the process of human fetal development and provide new and multi-organ information for understanding the possible roles of EZH2 during the whole fetal development. METHODS：33 human fetuses at 25 different gestational weeks and 1 neonatal sample were obtained through abortion or stillbirth. The organ or tissue including esophagus,stomach,small intestine,colon,liver, pancreas,parotid gland,thyroid,adrenal gland,lung,trachea,heart,large artery,kidney,bladder,uterus, ovary,testis,spleen,thymus,cerebrum were fixed,embedded,sectioned,then stained with hematoxylin and eosin to identify their morphological structures. Tissue microarrays were constructed and followed by immunohistochemical staining of EZH2. RESULTS：EZH2 was expressed in fetal organs investigated and exhibited different expression levels in different tissues and at various gestational ages. The positive staining of EZH2 protein was mainly located in the nucleus,as well as in the cytoplasm in some cells. CONCLUSION：EZH2 protein was generally expressed in human fetal organs and tissues and its expression exhibited tissue specificity and dependence on the stages of fetal development.